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描述
0.2 μm sterile filtered
suitable for 3D bioprinting applications
形狀
viscous liquid
包裝
1 ea of 10 mL
雜質
≤5 CFU/g Bioburden (Fungal)
≤5 CFU/g Bioburden (Total Aerobic)
顏色
colorless to pale yellow
pH值
6.5-7.5
應用
3D bioprinting
儲存溫度
2-8°C
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一般說明
3D bioprinting is the printing of biocompatible materials, cells, growth factors, and the other supporting materials necessary to yield functional complex living tissues. 3D bioprinting has been used to generate several different types of tissue such as skin, bone, vascular grafts, and cartilage structures. Based upon the desired properties, different materials and formulations can be used to generate both hard and soft tissues. While several 3D printing methods exist, due to the sensitivity of the materials used, extrusion-based methods with bioinks are most commonly employed.
應用
Gelatin methacryloyl (GelMA) is a polymerizable hydrogel material derived from natural extracellular matrix (ECM) components. Due to its low cost, abundance, and retention of natural cell binding motifs, gelatin has become a highly sought material for tissue engineering applications. Alginate is a naturally occurring polymer widely applied for bioprinting applications as its printability can be easily modified by altering the polymer density and crosslinking with the addition of calcium chloride (CaCl2). Alginate is often combined with gelatin to facilitate cell adhesion and differentiation. The addition of photocrosslinkable methacrylamide functional groups in GelMA allows the synthesis of biocompatible, biodegradable, and non-immunogenic hydrogels that are stable in biologically relevant conditions and promote cell adhesion, spreading, and proliferation. In addition to fast gelation, the methacrylamide functional group can also be used to control the hydrogel physical parameters such as pore size, degradation rate, and swell ratio. Temporal and spatial control of the crosslinking reaction can be obtained by adjusting the degree of functionalization and polymerization conditions, allowing for the fabrication of hydrogels with unique patterns, 3D structures, and morphologies. Gelatin methacrylate bioinks have been used for 3D bioprinting with high printing resolution, shape fidelity and cell viability. Gelatin methacrylate based bioinks have been used to bioprint osteogenic, chondrogenic, hepatic, adipogenic, vasculogenic, epithelial, endothelial, cardiac valve, skin, tumor and other tissues and constructs. Gelatin and alginate-containing bioinks have been used for bioprinting of 3D constructs with various cell types including human mesenchymal stem cells (hMSC), embryonic stem cells (ESC), human umbilical vein endothelial cells (HUVEC), fibroblasts, and cancer cells.
包裝
Product contains 10 ml of solution packaged in glass bottle.
其他說明
Important tips for optimal bioprinting results
Procedure
1. Prepare bioink solution: Warm TissueFab® - GelAlg-UV bioink in a water bath or incubator set to 37 °C for 30 minutes or until the bioink becomes fluid. Gently invert the bioink to make a homogeneous solution. DO NOT vortex or shake vigorously.
2. Prepare bioink-cell solution: Resuspend the cell pellet at the desired cell density with the bioink solution by gently pipetting up and down. Typical cell density for extrusion-based bioprinting is 1 to 5 x 106 cells/mL. Load the bioink-cell solution into the desired printer cartridge.
3. Bioprint: Cool the filled printer cartridge below 23 °C to induce gelation, using a temperature controlled printhead or place the cartridge at 4 °C for a few minutes. If print bed temperature control is available, set temperature to 20 °C. Follow the 3D printer manufacturer′s instructions. Load the print cartridge onto the 3D printer and print directly onto a Petri dish or into multi-well plates. Adjust the flow according to nozzle diameter, printing speed, printing pressure, and temperature. For optimal results, print under a gentle flow of 200 mM CaCl2 solution. A portable humidifier may be used to maintain the flow of the CaCl2 solution.
4. Crosslink: To photocrosslink, place the UV light source directly above the 3D-bioprinted structure and expose the structure to UV light (wavelength 365 nm). Use the appropriate distance settings and exposure times for your bioprinter. To chemically crosslink the printed structure, add 100mM CaCl2 in PBS for 1 minute. Rinse with PBS twice.
5. Culture cells: Culture the bioprinted tissue with appropriate cell culture medium following standard tissue culture procedures.
- Optimize printing conditions (e.g., nozzle diameter, printing speed, printing pressure, temperature, cell density) for the features of your 3D printer and your application.
- Reduce bubble formation. Air bubbles in bioink may hamper bioprinting. Carefully handle the bioink when you mix and transfer it to avoid bubble formation. Do not vortex or shake vigorously.
- UV light Crosslinking. Position the light source directly above the printed structure. Lower intensity light sources will require shorter distances and longer exposure times to complete crosslinking. Recommended conditions: Place an 800 mW/cm2 light source 8 cm above the printed structure and expose for 30 to 60 s.
Procedure
1. Prepare bioink solution: Warm TissueFab® - GelAlg-UV bioink in a water bath or incubator set to 37 °C for 30 minutes or until the bioink becomes fluid. Gently invert the bioink to make a homogeneous solution. DO NOT vortex or shake vigorously.
2. Prepare bioink-cell solution: Resuspend the cell pellet at the desired cell density with the bioink solution by gently pipetting up and down. Typical cell density for extrusion-based bioprinting is 1 to 5 x 106 cells/mL. Load the bioink-cell solution into the desired printer cartridge.
3. Bioprint: Cool the filled printer cartridge below 23 °C to induce gelation, using a temperature controlled printhead or place the cartridge at 4 °C for a few minutes. If print bed temperature control is available, set temperature to 20 °C. Follow the 3D printer manufacturer′s instructions. Load the print cartridge onto the 3D printer and print directly onto a Petri dish or into multi-well plates. Adjust the flow according to nozzle diameter, printing speed, printing pressure, and temperature. For optimal results, print under a gentle flow of 200 mM CaCl2 solution. A portable humidifier may be used to maintain the flow of the CaCl2 solution.
4. Crosslink: To photocrosslink, place the UV light source directly above the 3D-bioprinted structure and expose the structure to UV light (wavelength 365 nm). Use the appropriate distance settings and exposure times for your bioprinter. To chemically crosslink the printed structure, add 100mM CaCl2 in PBS for 1 minute. Rinse with PBS twice.
5. Culture cells: Culture the bioprinted tissue with appropriate cell culture medium following standard tissue culture procedures.
法律資訊
TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
其他客户在看
Liliang Ouyang et al.
Biofabrication, 8(3), 035020-035020 (2016-09-17)
3D cell printing is an emerging technology for fabricating complex cell-laden constructs with precise and pre-designed geometry, structure and composition to overcome the limitations of 2D cell culture and conventional tissue engineering scaffold technology. This technology enables spatial manipulation of
Wanjun Liu et al.
Advanced healthcare materials, 6(12) (2017-05-04)
Bioprinting is an emerging technique for the fabrication of 3D cell-laden constructs. However, the progress for generating a 3D complex physiological microenvironment has been hampered by a lack of advanced cell-responsive bioinks that enable bioprinting with high structural fidelity, particularly
Methacrylated gelatin and mature adipocytes are promising components for adipose tissue engineering.
Birgit Huber et al.
Journal of biomaterials applications, 30(6), 699-710 (2015-05-29)
In vitro engineering of autologous fatty tissue constructs is still a major challenge for the treatment of congenital deformities, tumor resections or high-graded burns. In this study, we evaluated the suitability of photo-crosslinkable methacrylated gelatin (GM) and mature adipocytes as components
Y Shi et al.
Biomedical materials (Bristol, England), 13(3), 035008-035008 (2018-01-09)
Three-dimensional bioprinting is an emerging technology for fabricating living 3D constructs, and it has shown great promise in tissue engineering. Bioinks are scaffold materials mixed with cells used by 3D bioprinting to form a required cell-laden structure. In this paper
B Duan et al.
Acta biomaterialia, 10(5), 1836-1846 (2013-12-18)
Tissue engineering has great potential to provide a functional de novo living valve replacement, capable of integration with host tissue and growth. Among various valve conduit fabrication techniques, three-dimensional (3-D) bioprinting enables deposition of cells and hydrogels into 3-D constructs
商品
Bioinks enable 3D bioprinting of tissue constructs for drug screening and transplantation; select suitable bioinks for specific tissue engineering.
Bioinks enable 3D bioprinting of tissue constructs for drug screening and transplantation; select suitable bioinks for specific tissue engineering.
生物墨水可3D生物打印形成功能组织结构，从而应用于药物筛选、疾病建模和体外移植。针对特定组织工程应用选择生物墨水和打印方法。
Bioinks enable 3D bioprinting of tissue constructs for drug screening and transplantation; select suitable bioinks for specific tissue engineering.
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