G003759

HPLC Analysis of Acrylamide in Fried Potato Chips on Discovery^® HS F5 after SPE using Discovery^® DSC-MCAX and DSC-18

application for SPE, application for HPLC

Properties

• technique(s): HPLC: suitable; solid phase extraction (SPE): suitable
• test parameters: sample preparation: SPE (Solid Phase Extraction); sample/matrix: Finely grind 2.0 g of potato chips, place in 20 mL vial and add 10 mL water. Mix using vortex until mixture forms a thick paste. (Place sample in centrifuge tubes and centrifuge at 16.1 rcf for 5 min. Extract aqueous portion of sample leaving oil layer and solids.); SPE tube/cartridge: Discovery DSC-MCAX, 300 mg/3 mL (52784-U); SPE tube/cartridge: Discovery DSC-18, 1g/6mL (52606-U); condition: connect a Discovery DSC-MCAX SPE tube to the top of a Discovery DSC-18 SPE tube using an SPE tube adapter, pass 1 mL methanol, then 1 mL DI water through both SPE tubes until the tubes are dry; sample addition: 1 mL of aqueous extract; washing: 1 mL DI water; elution: removing upper MCAX SPE tube, pass 2 mL methanol through lower DSC-18 SPE tube; eluate post-treatment: evaporate to dryness with nitrogen at 30 °C, reconstitute in 0.5 mL DI water; column: Discovery HS F5, 15 cm x 4.6 mm I.D., 3 μm particles (567507-U); mobile phase: 100% ultra-pure water; column temp.: 35 °C; detector: ESI+; injection: 5 μL
• suitability: application for HPLC; application for SPE
• application(s): food and beverages

Description

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany