RAB0385

Atrial Natriuretic Peptide EIA Kit

for serum, plasma, culture supernatant and cell lysates

• Synonym(s): Natriuretic peptides A, Prepronatriodilatin, ANP
• UNSPSC Code: 41116158
• NACRES: NA.32

Properties

• species reactivity: mouse, human, rat
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable; enzyme immunoassay: suitable
• input: sample type serum; sample type cell lysate; sample type plasma; sample type culture supernatant(s)
• assay range: inter-assay cv: <15%; intra-assay cv: <10%; sensitivity: 1.02 pg/mL; standard curve range: 0.1-1000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... NPPA(4878)

Description

General description

The ANP Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting ANP peptide based on the principle of Competitive Enzyme Immunoassay. EIA-ANP-1 targets the common sequence of human, mouse and rat, and thus may be used to detect ANP expression in all these species with high specificity and sensitivity.

Immunogen

Atrial Natriuretic Peptide, synthetic peptide

Application

Atrial Natriuretic Peptide EIA Kit has been used for the quantification of atrial natriuretic peptide (ANP) in cell culture.

For research use only. Not for use in diagnostic procedures.
Atrial Natriuretic Peptide EIA Kit has been used to measure ANP levels in diabetes mellitus patients.

Biochem/physiol Actions

Atrial natriuretic peptide (ANP) is a polypeptide hormone released in response to atrial dilation and it stimulates diuresis, natriuresis and vasorelaxation. ANP acts as an inhibitor of renin-angiotensin-aldosterone system (RAAS) pathway. It also has a major role in body fluid homeostasis and maintenance of blood pressure. Binding of ANP to receptors on target cell stimulate the production of cyclic guanosine monophosphate (GMP). Overexpression of ANP leads to congestive heart failure in the human. Experimental studies show that ANP can be used as a therapeutic for stimulating angiogenesis in ischemic tissues. N-terminal ANP prohormone functions as a biomarker for asymptomatic systolic left ventricular (LV) dysfunction, congestive heart failure and cardiovascular mortality.

Kit Components Also Available Separately

• RABHRP3 HRP-Streptavidin

Safety Information

• Pictograms: GHS05
• Signal Word: Warning
• Hazard Statements: H290
• Precautionary Statements: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class Code: 8A - Combustible corrosive hazardous materials