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Key Documents

P302-05

Sigma-Aldrich

Porcine Pulmonary Artery Endothelial Cells: PPAEC (Cryovial)

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

Porcine pulmonary artery (Normal healthy)

Quality Level

form

solid

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 38

morphology

Endothelial

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

diabetes; cardiovascular diseases

shipped in

dry ice

storage temp.

−196°C

General description

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PPAEC have been used in a pioneer study of endothelial cell motility, where it was determined that asymmetric dimethylarginine (ADMA), an inhibitor of nitric oxide production and a cardiovascular risk factor, decreased PKG-mediated phosphorylation of VASP, leading to downregulation of Rac1 and induction of stress fibers and focal adhesions via RhoA/ROCK dependent mechanism, leading to decreased cell motility, and increase in endothelial permeability, thus linking vascular repair and function with NO production (Wojciak-Stothard, 2007, 2009). PPAEC were also used to assess endothelial adhesion to decellularized porcine arteries in order to develop grafts for human vascular repair (Xiong, 2013).

Cell Line Origin

Artery

Application

cardiovascular function, screening of drug candidates, angiogenesis, vasodilator function, transport of deoxygenated bloood from heart to lungs

Components

Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO

Preparation Note

  • 1st passage, >500,000 cells in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO
  • Can be cultured at least 16 doublings

Subculture Routine

Please refer to the PPAEC Culture Protocol.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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