Clone 15 HL-60
98052918, human unknown/unspecified, Lymphoblastoid
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About This Item
UNSPSC Code:
41106514
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product name
Clone 15 HL-60, 98052918
biological source
human unknown/unspecified
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblastoid
products
Not specified
receptors
IL-5
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human caucasian promyelocytic leukaemia
Cell Line Description
The clone 15 HL-60 has been derived from HL-60 cells (Sigma Catalogue number. 98070106) that had been cultured at an elevated pH of 7.6 - 7.8 for 2 months. Clone 15 HL-60 was picked from soft agar being selected for the ability to differentiate to eosinophils after treatment with butyric acid. Although 80% of the cells will exhibit this differentiation pattern initially, reversion will occur during culture leading to a higher percentage reverting to neutrophilic differentiation. Expression of IL-5 receptors and eosinophil granule proteins has been found (eosinophil major basic protein (MBP), eosinophil peroxidase (EPO), eosinophilotactions protein (ECP) and eosinophil derived neurotoxin (EDN). In addition, chemotaxis to eosinophil specific eosinophilotactius has been described.
Application
Leukemia studies, oncogene expression, differentiation assays
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9 x 100,000 cells per ml; 5% CO2; 37°C. Immediately after resuscitation use medium with 20% foetal bovine serum (FBS) to aid establishment of growing culture. Reduce FBS concentration to 10% once cells are well established in
Other Notes
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Certificates of Analysis (COA)
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