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MAB2040

Sigma-Aldrich

Anti-Heparin/Heparan Sulfate Antibody, clone T320.11

clone T320.11, Chemicon®, from mouse

Synonym(s):

Anti-Heparan Sulfate, Clone T320.11 Anti-Heparin, Heparan Sulfate Detection

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

T320.11, monoclonal

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HSPG2(3339)

Specificity

Reacts with heparin/heparan sulfate. Low affinity cross-reactivity with DNA. It is reported that clone also cross reacts with dextran sulfate (Mahmood, 2002).

Application

Detect Heparin/Heparan Sulfate using this Anti-Heparin/Heparan Sulfate Antibody, clone T320.11 validated for use in ELISA, RIA & WB.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
Western blotting (recognizes heparan sulfate proteoglycan) 1:1000

EIA/RIA: Suggested concentration 1-10 μg/mL.

Optimal working dilutions must be determined by end user.

Target description

64 kDa

Physical form

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Widely expressed

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Emilie Barilleau et al.
BMC microbiology, 21(1), 153-153 (2021-05-23)
Salmonella can invade host cells via a type three secretion system called T3SS-1 and its outer membrane proteins, PagN and Rck. However, the mechanism of PagN-dependent invasion pathway used by Salmonella enterica, subspecies enterica serovar Typhimurium remains unclear. Here, we
Kyeoung-Hwa Kim et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 45(1), 37-53 (2018-01-10)
Previously, we found that silencing of growth arrest-specific gene 6 (Gas6) in oocytes impaired cytoplasmic maturation, resulting in failure of sperm chromatin decondensation (SCD) and pronuclear (PN) formation after fertilization. Thus, we conducted this study to determine the effect of
Obligatory role for endothelial heparan sulphate proteoglycans and caveolae internalization in catestatin-dependent eNOS activation.
Fornero, S; Bassino, E; Ramella, R; Gallina, C; Mahata, SK; Tota, B; Levi, R; Alloatti, G; Gallo, MP
BioMed Research International null
Varadarajan Sudhahar et al.
American journal of physiology. Cell physiology, 319(5), C933-C944 (2020-09-17)
Caveolin-1 (Cav-1) is a scaffolding protein and a major component of caveolae/lipid rafts. Previous reports have shown that endothelial dysfunction in Cav-1-deficient (Cav-1-/-) mice is mediated by elevated oxidative stress through endothelial nitric oxide synthase (eNOS) uncoupling and increased NADPH
Christina Guzzo et al.
PLoS pathogens, 9(12), e1003852-e1003852 (2014-01-05)
CD8+ T cells play a key role in the in vivo control of HIV-1 replication via their cytolytic activity as well as their ability to secrete non-lytic soluble suppressive factors. Although the chemokines that naturally bind CCR5 (CCL3/MIP-1α, CCL4/MIP- 1β

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