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204903

Sigma-Aldrich

Anti-Complement 5b-9 Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-Terminal Complement Complex, Anti-Membrane Attack Complex, Anti-MAC

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Protein A purified rabbit polyclonal antibody. Recognizes the C5b-C9 complement component complex.
Recognizes complement component complex Cb5-C9.
This Anti-Complement 5b-9 Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence, Paraffin Sections, Radial Immunodiffusion for the detection of Complement 5b-9.

Immunogen

Human
purified human SC5b-9 complex

Application

Frozen Sections (see application references)

Immunofluorescence (see comments)

Paraffin Sections (see comments, heat pre-treatment required)

Radial Immunodiffusion (use undiluted)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, pH 7.2.

Reconstitution

Following intial thaw, aliquot and freeze (-20°C).

Other Notes

By immunodiffusion the antibody is monospecific for C5b-9 complex in purified form or present in cobra venom factor activated human serum. There is no reactivity vs. non-activated normal human serum or plasma. Also reported to work for immunofluorescence. This antibody has been shown to work for formalin-fixed paraffin sections; use of citrate buffer and a pressure cooker for 1 min is required for antigen retrieval. Antibody should be titrated for optimal results in individual systems.
Schafer, H., et al. 1986. J. Immunol.137, 1945.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jean F Regal et al.
Molecular immunology, 78, 38-47 (2016-09-03)
Preeclampsia is characterized by development of hypertension during pregnancy and reduced placental perfusion. Previous studies in a rat model of placental ischemia-induced hypertension demonstrated that inhibiting complement activation attenuated increased maternal blood pressure with C3a and C5a identified as the
Melina V Jones et al.
Journal of immunology research, 2018, 9034695-9034695 (2019-01-17)
To reduce immune-mediated damage in a rat model of neuromyelitis optica (NMO) by blocking neutrophil migration using SCH527123, a drug that inhibits CXCR2. Neuromyelitis optica is a relapsing autoimmune disease that preferentially targets the optic nerves and spinal cord leading
E Panayiotou et al.
Biochemistry and biophysics reports, 8, 48-54 (2017-09-29)
Penetrance and age of onset of ATTRV30M amyloidotic neuropathy varies significantly among different populations. This variability has been attributed to both genetic and environmental modifiers. We studied the effect of genetic background on phenotype in two lines of transgenic mice
Xiao Han et al.
International journal of molecular sciences, 18(1) (2017-01-21)
The initiator of extrinsic coagulation, tissue factor (TF), and its non-coagulant isoform alternatively spliced TF (asTF) are closely associated with tumor development. In the tumor microenvironment, the role of TF-induced coagulation in tumor progression remains to be fully elucidated. Using
Ting-Ting Gao et al.
International journal of ophthalmology, 8(4), 675-680 (2015-08-27)
To investigate the expression of complement factors in the posterior scleral fibroblasts of guinea pigs with negative lens-defocused myopia. Eighteen guinea pigs were assigned randomly to two groups: the negative lens-defocused group (NLD group, n=9) and the normal control without

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