Skip to Content
Merck
  • Using nonrandom two-liquid model for solvent system selection in counter-current chromatography.

Using nonrandom two-liquid model for solvent system selection in counter-current chromatography.

Journal of chromatography. A (2014-06-22)
Da-Bing Ren, Yan-Hua Qin, Yong-Huan Yun, Hong-Mei Lu, Xiao-Qing Chen, Yi-Zeng Liang
ABSTRACT

Selection of an appropriate solvent system is of great importance for a successful counter-current chromatography separation. In this work, the nonrandom two-liquid (NRTL) model, a thermodynamic method, was used for predicting the partition coefficient based on a few measured partition coefficients. The NRTL method provides quite satisfactory results for model solutes in first correlating measured partition coefficient in a few representative biphasic liquid systems and then successfully predicting partition coefficient in other two-phase liquid systems. According to the predicted partition coefficient, a suitable solvent system can be screened. Assisted with the NRTL method, the solvent system composed of hexane/ethyl acetate/methanol/water (1:4:1:4, v/v) was rapidly screened for the successful separation of two major compounds with high purity from Malus hupehensis leaves. The results demonstrated that the NRTL model can offer a simple and practical strategy to estimate partition coefficients in support of CCC solvent system selection, which will significantly minimize the experimental efforts and cost involved in solvent system selection.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-(p-Chlorophenoxy)-2-methylpropionic acid, 97%
Sigma-Aldrich
Acetylsalicylic acid, analytical standard
Sigma-Aldrich
Acetylsalicylic acid, ≥99.0%
Sigma-Aldrich
Coumarin, ≥99% (HPLC)
Acetylsalicylic acid, European Pharmacopoeia (EP) Reference Standard
Supelco
Clofibric acid, analytical standard
Coumarin, primary reference standard
Acetylsalicylic acid for peak identification, European Pharmacopoeia (EP) Reference Standard
Coumarin, European Pharmacopoeia (EP) Reference Standard
Supelco
Coumarin, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Aspirin, meets USP testing specifications
Supelco
Aspirin (Acetyl Salicylic Acid), Pharmaceutical Secondary Standard; Certified Reference Material
USP
Aspirin, United States Pharmacopeia (USP) Reference Standard