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Expression of Myosin 5a splice variants in murine stomach.

Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society (2021-05-04)
Josephine A Carew, Vivian Cristofaro, Nicolas A Siegelman, Raj K Goyal, Maryrose P Sullivan
ABSTRACT

The motor protein, Myosin 5a (Myo5a) is known to play a role in inhibitory neurotransmission in gastric fundus. However, there is no information regarding the relative expression of total Myo5a, or of its alternative exon splice variants, across the stomach. This study investigated the differential distribution of Myo5a variants expressed within distinct anatomical regions of murine stomach. The distribution of Myo5a protein and mRNA in the stomach was assessed by immunofluorescence microscopy and fluorescent in situ hybridization. Quantitative PCR, restriction enzyme analysis, and electrophoresis were used to identify Myo5a splice variants and quantify their expression levels in the fundus, body, antrum, and pylorus. Myo5a protein colocalized with βIII-Tubulin in the myenteric plexus, and with synaptophysin in nerve fibers. Total Myo5a mRNA expression was lower in pylorus than in antrum, body, or fundus (p < 0.001), which expressed equivalent amounts of Myo5a. However, Myo5a splice variants were differentially expressed across the stomach. While the ABCE splice variant predominated in the antrum and body regions, the ACEF/ACDEF variants were enriched in fundus and pylorus. Myo5a splice variants varied in their relative expression across anatomically distinguishable stomach regions and might mediate distinct physiological functions in gastric neurotransmission.

MATERIALS
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Brand
Product Description

Sigma-Aldrich
Anti-Myosin Va (LF-18) antibody produced in rabbit, ~0.4 mg/mL, affinity isolated antibody, buffered aqueous solution