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  • Leukemia inhibitory factor, glial cell line-derived neurotrophic factor, and their receptor expressions following muscle crush injury.

Leukemia inhibitory factor, glial cell line-derived neurotrophic factor, and their receptor expressions following muscle crush injury.

Muscle & nerve (1999-10-08)
K Kami, Y Morikawa, Y Kawai, E Senba
ABSTRACT

Using in situ hybridization histochemistry, we characterized the spatiotemporal gene expression patterns of leukemia inhibitory factor (LIF) and glial cell line-derived neurotrophic factor (GDNF), and their receptor components (LIFR, GFR-alpha1, RET) induced in muscle cells, intramuscular nerves, and motoneurons in the regeneration processes of both muscle cells and nerves following muscle contusion. Muscle contusion induced upregulation of GDNF and GFR-alpha1 mRNAs in Schwann cell-like cells in the intramuscular nerves and of LIFR mRNA in damaged muscle cells. LIFR, GFR-alpha1, and RET mRNA expressions in motoneurons were upregulated following muscle contusion. Muscle contusion also induced more rapid, prominent transactivations of GFR-alpha1 and RET genes in motoneurons than did sciatic nerve axotomy. These findings suggest that rapid and prominent upregulation of the receptor components for LIF and GDNF in motoneurons is important for the regeneration of intramuscular motor nerves damaged by muscle contusion.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Leukemia Inhibitory Factor human, 5 µg, human recombinant LIF protein, expressed in E. coli, suitable for stem cell culture
Sigma-Aldrich
Leukemia Inhibitory Factor human, 10 µg, human recombinant LIF protein, expressed in E. coli, suitable for stem cell culture
Sigma-Aldrich
Leukemia Inhibitory Factor from mouse, 10 µg, mouse recombinant LIF protein, expressed in E. coli, suitable for stem cell culture
Sigma-Aldrich
Leukemia Inhibitory Factor from mouse, 5 µg, mouse recombinant LIF protein, expressed in E. coli, suitable for stem cell culture