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Key Documents

P1259

Sigma-Aldrich

Phytase from wheat

≥0.01 unit/mg solid

Synonym(s):

6-Phytase, Phytate 6-phosphatase, myo-Inositol-hexakisphosphate 6-phosphohydrolase

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

form

powder

specific activity

≥0.01 unit/mg solid

storage temp.

−20°C

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General description

Phytase catalyzes the hydrolysis of phytic acid to inositol and free orthophosphate.

Application

Phytase has been used in a study to quantify the number of feedstuffs showing significant phytase activity. It has also been used in a study to investigate the effect of adjusting the pH to 5.0 (optimum for wheat phytase) during whole wheat bread making on phytase activity.

Biochem/physiol Actions

Wheat phytase can degrade inositol phosphate-6 and 5 at a pH of 4 and 5.

Quality

Crude

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphorus from 1.5 mM phytate per min at pH 5.15 at 55 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Total phosphorus, phytate-phosphorus and phytase activity in plant feedstuffs
Eeckhout, W. and M. Paepe
Anim. Feed Sci. Technol., 47, 19-29 (1994)
J C González-Vega et al.
Journal of animal science, 91(10), 4807-4816 (2013-08-15)
An experiment was conducted to test the hypothesis that values for apparent total tract digestibility (ATTD) of Ca in pigs are influenced by endogenous Ca lost from the gastrointestinal tract. The objective was to determine the endogenous loss of Ca
M P Létourneau-Montminy et al.
Animal : an international journal of animal bioscience, 6(10), 1590-1600 (2012-10-04)
Optimizing phosphorus (P) utilization in pigs requires improving our capacity to predict the amount of P absorbed and retained, with the main modulating factors taken into account, as well as precisely determining the P requirements of the animals. Given the
Katrine Pontoppidan et al.
Archives of animal nutrition, 66(6), 431-444 (2012-10-27)
Phytases (EC 3.1.3) are widely used in animal feed to increase the availability of phosphorus and decrease the anti nutritive effect of myo-inositol hexakisphosphate (InsP₆). The aim of this work was to investigate the stereospecific degradation of InsP₆ in vitro
The phytase of wheat.
F G PEERS
The Biochemical journal, 53(1), 102-110 (1953-01-01)

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