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Key Documents

H2387

SAFC

Hanks′ Balanced Salts

Modified, without calcium chloride, magnesium sulfate and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

HBSS

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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General description

Balanced salt solution (BSS) provides cells with water, bulk inorganic ions, and buffering system to maintain the normal cell metabolism and physiological range (pH 7.2-7.6), respectively. It also acts as an irrigating, diluting, and transporting agent and maintains the intracellular and extracellular osmotic balance. BSS also provides energy sources such as glucose for cell metabolism.

Application

Hanks′ Balanced Salt has been used:

  • to culture primary hippocampal neuron cells from rats
  • to collect the rat brains
  • to culture rat primary hepatocytes

Quantity

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Other Notes

The pH is adjusted after addition of sodium bicarbonate.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Seo Yeon Lee et al.
Polymers, 12(2) (2020-02-20)
Herein, we describe precisely a covalent modification of pure magnesium (Mg) surface and its application to induce in vitro osteogenic differentiation. The new concept of a chemical bonding method is proposed for developing stable chemical bonds on the Mg surface
Yu-Kyoung Kim et al.
Scientific reports, 8(1), 13264-13264 (2018-09-07)
A polymer coating as polycaprolactone (PCL) is applied to improve the initial corrosion resistance of biodegradable magnesium. In addition, plasma electrolytic oxidation (PEO) is performed to increase adhesion between the polymer and the metal. However, when a complex-shaped material such
Yu Bai et al.
Journal of materials science. Materials in medicine, 27(2), 31-31 (2015-12-26)
Au nanoparticles (AuNPs) are successfully assembled on TiO2 nanotube (TN) arrays through electrochemical deposition technology to improve the surface characteristics of TN arrays as an implant material. The loading amount of AuNPs can be controlled by adjusting the deposition time
Mostafa M H Ibrahim et al.
ASN neuro, 12, 1759091420974134-1759091420974134 (2020-11-13)
Norepinephrine (NE) control of hypothalamic gluco-regulation involves astrocyte-derived energy fuel supply. In male rats, exogenous NE regulates astrocyte glycogen metabolic enzyme expression in vivo through 5'-AMP-activated protein kinase (AMPK)-dependent mechanisms. Current research utilized a rat hypothalamic astrocyte primary culture model
Xiaojun Huang et al.
Progress in biophysics and molecular biology, 156, 3-13 (2020-08-08)
Cryo-electron microscopy (cryoEM) has become one of the most important approach for structural biology. However, barriers are still there for an increased successful rate, a better resolution and improved efficiency from sample preparation, data collection to image processing. CryoEM sample

Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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