01818
Acylase I from Aspergillus melleus
powder, brown, >0.5 U/mg
Synonym(s):
Acylase ‘Amano’, Aminoacylase
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About This Item
Recommended Products
form
powder
specific activity
>0.5 U/mg
color
brown
storage temp.
2-8°C
General description
Acylase I belongs to the aminoacylase family of enzymes.
Application
Acylase I from Aspergillus melleus has been used to catalyze the Mannich reaction.
Biochem/physiol Actions
Acylase I plays an important role in the amino acid metabolism of organisms. It also plays a role in the acylation of primary and secondary alcohols. Acylase I is involved in alcoholysis.
Unit Definition
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-acetyl-L-methionine per minute at pH 8.0 and 37°C
Analysis Note
Enzyme activity: the optimum temperature is 40-45°C, the optimum pH is 8.0 (stable form pH 6-10). The enzyme is activated by CoCl2 in the range of 10-4 to 10-3 M.
Other Notes
Resolution of acetyl amino acids
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Tetrahedron, 12(17), 2447-2455 (2001)
Enzyme-catalyzed asymmetric Mannich reaction using acylase from Aspergillus melleus
Journal of Molecular Catalysis. B, Enzymatic, 111, 16-20 (2015)
Journal of Labelled Compounds & Radiopharmaceuticals, 29, 867-867 (1991)
Physicochemical and catalytic properties of acylase I from aspergillus melleus immobilized on amino- and carbonyl-grafted stober silica
Biotechnology Progress, 767-777 (2018)
Antonie van Leeuwenhoek, 94(4), 527-532 (2008-07-31)
A total of 190 Campylobacter spp. isolates, of which 34 gave the result of very weak activity, and 156 gave the negative activity in the test for hippurate hydrolysis were characterized. The genomic DNA was isolated from a fresh culture
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