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Key Documents

ABS54

Sigma-Aldrich

Anti-AS160 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Acrg embryonic lethality minimal region ortholog, Akt substrate of 160 kDa, TBC (Tre-2, BUB2, CDC16) domain-containing protein, TBC1 domain family, member

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

species reactivity (predicted by homology)

rat (based on 100% sequence homology), canine (based on 100% sequence homology)

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_001074747

UniProt accession no.

Q8BYJ6

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TBC1D4(9882)
mouse ... Tbc1D4(210789)

General description

AS160 (Akt Substrate of 160 kDa, Akt1S1), Rab GAP (GTPase activating protein), is phosphorylated on multiple sites by Akt in response to insulin in adipocytes and contraction in muscles. AS160 is the long sought after link between the upstream insulin signaling through insulin/PI3K/Akt and GLUT4 vessicle translocation to the cell membrane. It has 6 Akt phosphorylation consensus sequences that are believed to become phosphorylated upon insulin stimulation. This phosphorylation is required for translocation of GLUT4-containing vesicles to the cell membrane1. A mutation of two or more of these phosphorylation sites results in a dramatic decrease in AS160 activity and insulin-stimulated GLUT4 redistribution at the cell membrane2. AS160 has also shown to be important for GLUT4 expcytosis2.

Specificity

Chimpanzee, equine, and ox (90% sequence homology).
This antibody recognizes AS160 at the C-terminus.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of mouse AS160.

Application

Anti-AS160 Antibody detects level of AS160 & has been published & validated for use in WB & IP.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected AS160 on 10 µg of HEPG2 cell lysate.

Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated AS160 from HEPG2 lysate.

Quality

Evaluated by Western Blot in HEPG2 cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected AS160 on 10 µg of HEPG2 cell lysate.

Target description

~ 160 kDa

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEPG2 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Haiyan Wang et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 71(3), 323-332 (2015-09-06)
Exercise and calorie restriction (CR) can each improve insulin sensitivity in older individuals, but benefits of combining these treatments on skeletal muscle insulin signaling and glucose uptake are poorly understood, especially in predominantly slow-twitch muscles (eg, soleus). Accordingly, our purpose
Lipid mixtures containing a very high proportion of saturated fatty acids only modestly impair insulin signaling in cultured muscle cells.
Newsom, SA; Everett, AC; Park, S; Van Pelt, DW; Hinko, A; Horowitz, JF
Testing null
Haiyan Wang et al.
American journal of physiology. Endocrinology and metabolism, 315(5), E859-E871 (2018-08-22)
A single exercise session can increase insulin-stimulated glucose uptake (GU) by skeletal muscle, concomitant with greater Akt substrate of 160 kDa (AS160) phosphorylation on Akt-phosphosites (Thr642 and Ser588) that regulate insulin-stimulated GU. Recent research using mouse skeletal muscle suggested that
Haiyan Wang et al.
Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme, 46(6), 685-689 (2021-03-26)
One exercise session can elevate insulin-stimulated glucose uptake (GU) by skeletal muscle, but it is uncertain if this effect is accompanied by altered membrane cholesterol content, which is reportedly inversely related to insulin-stimulated GU. Muscles from sedentary (SED) or exercised
Naveen Sharma et al.
American journal of physiology. Endocrinology and metabolism, 308(7), E603-E612 (2015-02-12)
Either calorie restriction [CR; consuming 60-65% of ad libitum (AL) intake] or acute exercise can independently improve insulin sensitivity in old age, but their combined effects on muscle insulin signaling and glucose uptake have previously been unknown. Accordingly, we assessed
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