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  • Cellular and molecular properties of neural progenitors in the developing mammalian hypothalamus.

Cellular and molecular properties of neural progenitors in the developing mammalian hypothalamus.

Nature communications (2020-08-15)
Xin Zhou, Suijuan Zhong, Honghai Peng, Jing Liu, Wenyu Ding, Le Sun, Qiang Ma, Zeyuan Liu, Ruiguo Chen, Qian Wu, Xiaoqun Wang
ABSTRACT

The neuroendocrine hypothalamus is the central regulator of vital physiological homeostasis and behavior. However, the cellular and molecular properties of hypothalamic neural progenitors remain unexplored. Here, hypothalamic radial glial (hRG) and hypothalamic mantle zone radial glial (hmRG) cells are found to be neural progenitors in the developing mammalian hypothalamus. The hmRG cells originate from hRG cells and produce neurons. During the early development of hypothalamus, neurogenesis occurs in radial columns and is initiated from hRG cells. The radial glial fibers are oriented toward the locations of hypothalamic subregions which act as a scaffold for neuronal migration. Furthermore, we use single-cell RNA sequencing to reveal progenitor subtypes in human developing hypothalamus and characterize specific progenitor genes, such as TTYH1, HMGA2, and FAM107A. We also demonstrate that HMGA2 is involved in E2F1 pathway, regulating the proliferation of progenitor cells by targeting on the downstream MYBL2. Different neuronal subtypes start to differentiate and express specific genes of hypothalamic nucleus at gestational week 10. Finally, we reveal the developmental conservation of nuclear structures and marker genes in mouse and human hypothalamus. Our identification of cellular and molecular properties of neural progenitors provides a basic understanding of neurogenesis and regional formation of the non-laminated hypothalamus.

MATERIALS
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Product Description

Sigma-Aldrich
Anti-TTYH1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Pricing and availability is not currently available.
Sigma-Aldrich
Anti-FAM107A antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Pricing and availability is not currently available.
Sigma-Aldrich
Cell dissociation sieve - tissue grinder kit, tissue grinder homogenizer kit, stainless steel, autoclavable, 1/kit
Pricing and availability is not currently available.
Sigma-Aldrich
Anti-NeuN Antibody, clone A60, clone A60, Chemicon®, from mouse
Pricing and availability is not currently available.