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  • Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells.

Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells.

Communications biology (2020-03-15)
Harmonie Perdreau-Dahl, Cinzia Progida, Stefan J Barfeld, Hanne Guldsten, Bernd Thiede, Magnus Arntzen, Oddmund Bakke, Ian G Mills, Stefan Krauss, J Preben Morth
ABSTRACT

Sjögren syndrome/scleroderma autoantigen 1 (SSSCA1) was first described as an auto-antigen over-expressed in Sjögren's syndrome and in scleroderma patients. SSSCA1 has been linked to mitosis and centromere association and as a potential marker candidate in diverse solid cancers. Here we characterize SSSCA1 for the first time, to our knowledge, at the molecular, structural and subcellular level. We have determined the crystal structure of a zinc finger fold, a zinc ribbon domain type 2 (ZNRD2), at 2.3 Å resolution. We show that the C-terminal domain serves a dual function as it both behaves as the interaction site to Tankyrase 1 (TNKS1) and as a nuclear export signal. We identify TNKS1 as a direct binding partner of SSSCA1, map the binding site to TNKS1 ankyrin repeat cluster 2 (ARC2) and thus define a new binding sequence. We experimentally verify and map a new nuclear export signal sequence in SSSCA1.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Pentachloroethane, 95%
Sigma-Aldrich
Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da
Sigma-Aldrich
MISSION® esiRNA, targeting human TNKS2
Sigma-Aldrich
MISSION® esiRNA, targeting human TNKS