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318744

Sigma-Aldrich

Bromophenol Blue solution

0.04 wt. % in H2O

Synonym(s):

3′,3′′,5′,5′′-Tetrabromophenolsulfonephthalein, Bromophenol Blue sodium salt

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About This Item

Empirical Formula (Hill Notation):
C19H9Br4NaO5S
CAS Number:
Molecular Weight:
691.94
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

form

liquid

Quality Level

concentration

0.04 wt. % in H2O

technique(s)

titration: suitable

pH

3.0-4.6, yellow to blue

density

0.985 g/mL at 25 °C

λmax

589 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O

InChI

1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1

InChI key

TWKDJMRHSLWRFI-UHFFFAOYSA-M

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General description

Bromophenol Blue is a tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Rapid transfer of DNA from agarose gels to nylon membranes.
Reed KC and Mann DA.
Nucleic Acids Research, 13, 7207-7221 (1985)
Detection of erythrocyte membrane proteins, sialoglycoproteins, and lipids in the same polyacrylamide gel using a double-staining technique.
Dzandu JK, et.al.
Proceedings of the National Academy of Sciences of the USA, 81, 1733-1737 (1984)
Detection of erythrocyte membrane proteins, sialoglycoproteins, and lipids in the same polyacrylamide gel using a double-staining technique.
Dzandu JK, et al.
Proceedings of the National Academy of Sciences of the USA, 81, 1733-1737 (1984)

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