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  • Interfacial properties and critical micelle concentration of lysophospholipids.

Interfacial properties and critical micelle concentration of lysophospholipids.

Biochemistry (1989-06-13)
R E Stafford, T Fanni, E A Dennis
ABSTRACT

The critical micelle concentration (cmc) of several lysophospholipids and of a lysophospholipid analogue was determined from surface tension measurements using the maximum bubble pressure method and/or 31P NMR. The use of the maximum bubble pressure method has now been extended to micromolar concentrations of surfactant, and the experimental parameters that effect its use have been explored. Surface activity was found to vary with changes in the chain length and in the headgroup polarity of the lysophospholipid. The cmc's for 1-decanoyl-, 1-dodecanoyl-, 1-tetradecanoyl-, and 1-hexadecanoyl-sn-glycero-3-phosphocholine are 7.0, 0.70, 0.070, and 0.007 mM, respectively. The cmc's for 1-decanoyl- and 1-dodecanoyl-sn-glycero-3-phosphoethanolamine are 4.4 and 0.33 mM, respectively. The cmc for dodecylphosphocholine, a lysophospholipid analogue, was found to be 1.1 mM. The cmc's for 1-tetradecanoyl- and 1-hexadecanoyl-sn-glycero-3-phosphoglycerol were found to be 3.0 and 0.60 mM, respectively, in pure water. In 0.1 M Tris-HCl (pH = 8.0), their cmc's are 0.16 and 0.018 mM, respectively. Surface tension and adsorption density values determined at the cmc are reported for each compound. The relationship of dynamic surface tension and lipid purity is discussed. These studies provide information about the micellization and interfacial properties of several biologically important lysophospholipids.

MATERIALS
Product Number
Brand
Product Description

Avanti
15:0 Lyso PC, 1-pentadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine, powder
Avanti
06:0 Lyso PC, 1-hexanoyl-2-hydroxy-sn-glycero-3-phosphocholine, powder
Avanti
16:0 Lyso PC, 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine, chloroform
Avanti
16:0 Lyso PC, Avanti Research - A Croda Brand
Avanti
17:0 Lyso PC, 1-heptadecanoyl-2-hydroxy-sn-glycero-3-phosphocholine, chloroform
Avanti
17:0 Lyso PC, Avanti Research - A Croda Brand
Avanti
11:0 Lyso PC, 1-undecanoyl-2-hydroxy-sn-glycero-3-phosphocholine, powder
Avanti
26:0 Lyso PC, 1-hexacosanoyl-2-hydroxy-sn-glycero-3-phosphocholine, powder
Avanti
10:0 Lyso PC, 1-decanoyl-2-hydroxy-sn-glycero-3-phosphocholine, powder
Avanti
08:0 Lyso PC, 1-octanoyl-2-hydroxy-sn-glycero-3-phosphocholine, powder
Avanti
16:0 Lyso PG, 1-palmitoyl-2-hydroxy-sn-glycero-3-phospho-(1′-rac-glycerol) (sodium salt), powder
Avanti
14:0 Lyso PC, 1-myristoyl-2-hydroxy-sn-glycero-3-phosphocholine, chloroform
Avanti
09:0 Lyso PC, 1-nonanoyl-2-hydroxy-sn-glycero-3-phosphocholine, powder
Avanti
14:0 Lyso PC, 1-myristoyl-2-hydroxy-sn-glycero-3-phosphocholine, powder
Avanti
18:1 Lyso PC, Avanti Research - A Croda Brand
Avanti
18:1 Lyso PC, 1-oleoyl-2-hydroxy-sn-glycero-3-phosphocholine, chloroform