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R9388

Sigma-Aldrich

Retinol Binding Protein from human urine

buffered aqueous solution

Synonym(s):

CRBP, Cellular retinol binding protein, RBP, RBP-1, Retinol Binding Protein I

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About This Item

MDL number:
MFCD00165761
UNSPSC Code:
12352202
NACRES:
NA.56

biological source

human urine

Quality Level

200

Assay

≥85% (SDS-GE)

form

buffered aqueous solution

technique(s)

cell culture | embryo: suitable
transfection: suitable

UniProt accession no.

P09455

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... RBP1(5947)

General description

Retinol Binding Protein (RBP) is a member of lipocalin superfamily. The normal concentration of RBP in serum is 40 to 50 μg/mL and has a molecular weight of 21 kDa. It has a turnover rate of 4 to 24 h. RBP is produced in the liver in response to retinol binding trigger. It is also produced in kidney, sertoli cells of testis. RBP contains a single globular domain and a highly conserved N-terminal region. It has eight antiparallel β sheets in the centre which binds retinol. In human chromosome, the gene encoding RBP is localised at 3q23.

Application

Retinol Binding Protein from human urine has been used:
  • as one of the protein in microinjecting mesencephalic cavity of chicken eggs to monitor embryonic cerebrospinal fluid/embryonic serum protein transfer
  • as an additive in basal medium for RNA transfection experiments
  • in the antibody-derivatized affinity pipettes preparation for the capture of affinity proteins

Biochem/physiol Actions

Inflammation induced by lipopolysaccharide (LPS) exposure caused a reduction in the level of retinol binding protein produced by the liver.
Retinol Binding Protein (RBP) is a specific protein for the transport of vitamin A. The apo-RBP, formed after delivering retinol to the tissues is catabolized by the kidney. The plasma concentration of RBP is less in diabetes mellitus patients. RBP binds to the carrier protein transerythrin, to prevent its loss during glomerular filtration. RBP is implicated in initial neurogenesis and neuroepithelial cell proliferation. RBP is responsible for blepharophimosis syndrome (BPES).

Physical form

Solution in 10 mM sodium phosphate, pH 7.4, 150 mM NaCl, and 0.05% NaN3

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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F J Rosales et al.
Journal of lipid research, 37(5), 962-971 (1996-05-01)
The acute inflammatory response to tissue injury and infection is associated with low concentrations of plasma retinol and its specific transport proteins, retinol-binding protein (RBP) and transthyretin (TTR). To examine the kinetics and mechanism of hyporetinemia, we have induced acute
Assignment of the cellular retinol-binding protein 1 gene (RBP1) and of the coatomer beta' subunit gene (COPB2) to human chromosome band 3q23 by in situ hybridization
De Baere E, et al.
Cytogenetics and Cell Genetics, 82, 226-227 (1998)
A blood-CSF barrier function controls embryonic CSF protein composition and homeostasis during early CNS development
Parvas M, et al.
Developmental Biology, 321(1), 51-63 (2008)
Laboratory Tests for the Assessment of Nutritional Status, Second Edition (1999)
Vitamin A in Health and Disease, 87-95 (1994)
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