I9274

Invertase from baker′s yeast (S. cerevisiae)

200-300 units/mg solid

• Synonym(s): β-D-Fructofuranosidase, β-D-Fructofuranoside fructohydrolase, Saccharase
• CAS Number: 9001-57-4
• Enzyme Commission number: 3.2.1.26 (BRENDA, IUBMB)
• EC Number: 232-615-7
• MDL number: MFCD00131410
• UNSPSC Code: 12164500
• NACRES: NA.21

Properties

• Quality Level: 100
• specific activity: 200-300 units/mg solid
• technique(s): photometry: suitable
• application(s): food and beverages; general analytical
• foreign activity: α-glucosidase (maltase), phosphoglucomutase, phosphoglucose isomerase ≤0.01%; 6-phosphogluconic dehydrogenase, α-galactosidase, β-galactosidase, and NADH oxidase ≤0.001%
• storage temp.: 2-8°C

Description

General description

Invertase is encoded by the SUC2 (Saccharomyces cerevisiae S288C) gene. In yeast this enzyme exist in: glycosylated (secreted) and non-glycosylated (intracellular) form. The external invertase is composed of carbohydrate (50%) and 60,000 D subunits.

Application

Invertase from baker′s yeast (S. cerevisiae) has been used:

• in liquid media (YES) and is used to determine the invertase activity
• supplemented to the reaction mixture in the extraction and analysis of sugars and starch
• for sucrose hydrolysis

Used in the production of confectionary foods and artificial honey.

Biochem/physiol Actions

Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.

Unit Definition

One unit will hydrolyze 1.0 μmole sucrose to glucose and fructose per minute at pH 4.6 at 25 °C.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves