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  • Multiple isoforms of human microtubule-associated protein tau: sequences and localization in neurofibrillary tangles of Alzheimer's disease.

Multiple isoforms of human microtubule-associated protein tau: sequences and localization in neurofibrillary tangles of Alzheimer's disease.

Neuron (1989-10-01)
M Goedert, M G Spillantini, R Jakes, D Rutherford, R A Crowther
ABSTRACT

We have determined the sequences of isoforms of human tau protein, which differ from previously reported forms by insertions of 29 or 58 amino acids in the amino-terminal region. Complementary DNA cloning shows that the insertions occur in combination with both three and four tandem repeats. RNAase protection assays indicate that transcripts encoding isoforms with the insertions are expressed in an adult-specific manner. Transcripts encoding four tandem repeats are also expressed in an adult-specific manner, whereas mRNAs encoding three tandem repeats are expressed throughout life, including in fetal brain. The levels of transcripts encoding the 29 or 58 amino acid inserts were not significantly changed in cerebral cortex from patients with Alzheimer's disease. Antisera raised against synthetic peptides corresponding to these different human tau isoforms demonstrate that multiple tau protein isoforms are incorporated into the neurofibrillary tangles of Alzheimer's disease.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Tau-410 human, recombinant, expressed in E. coli, ≥85% (SDS-PAGE), lyophilized powder
Sigma-Aldrich
Tau-383 human, recombinant, expressed in E. coli, ≥90% (SDS-PAGE), lyophilized powder
Sigma-Aldrich
Anti-Tau-1 Antibody, clone PC1C6, clone PC1C6, Chemicon®, from mouse
Sigma-Aldrich
Tau-412 human, recombinant, expressed in E. coli, ≥90% (SDS-PAGE), lyophilized powder
Sigma-Aldrich
Tau-441 human, recombinant, expressed in E. coli, ≥90% (SDS-PAGE), lyophilized powder
Sigma-Aldrich
Tau-352 human, recombinant, expressed in E. coli, ≥90% (SDS-PAGE), lyophilized powder
Sigma-Aldrich
Tau-381 human, recombinant, expressed in E. coli, ≥90% (SDS-PAGE), lyophilized powder