The effect of Ti anodized nano-foveolae structure on preosteoblast growth and osteogenic gene expression.

The effect of Ti anodized nano-foveolae structure on preosteoblast growth and osteogenic gene expression.

Journal of nanoscience and nanotechnology (2014-04-18)

Wei-Qiang Yu, Ling Xu, Fu-Qiang Zhang

ABSTRACT

The TiO2 nanotubes by anodization have been extensively studied for medical implant and orthopedic applications because of enhancing bone development. In the present study, a new nano-foveolae structure verified by SEM and AFM was prepared by simulating the nanotubes exfoliation from anodized Ti. MC3T3-E1 preosteoblasts were used to investigate the effects of new nanoscale surface on cell spreading, proliferation, and osteogenic gene expression. The new nano-foveolae structure supported preosteoblast better spreading, more filopodiaes shown by SEM (4 h), and lower proliferation (72 h) than the smooth Ti. By two weeks, the new nanoscale surface induced higher expression of osteogenic markers alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN), and collagen I (COL I) with real-time RT-PCR compared to the control group. The results of this study suggest that the new nano-foveolae structure provides a favorable surface for functions of bone cells.

MATERIALS

Product Number

Brand

Product Description

305812

Sigma-Aldrich

Titanium, 5-10 mm, ≥99.99% trace metals basis (purity exclusive of Na and K content)

IRMM531B

Titanium, IRMM^®, certified reference material, 0.5 mm foil

IRMM531C

Titanium, IRMM^®, certified reference material, 0.5 mm wire

GF62977540

Titanium, tube, 200mm, outside diameter 2.03mm, inside diameter 1.63mm, wall thickness 0.2mm, annealed, 99.6+%

GF62676759

Titanium, rod, 200mm, diameter 2mm, as drawn, 99.99+%

GF25290581

Titanium, tube, 1000mm, outside diameter 25.4mm, inside diameter 23.62mm, wall thickness 0.89mm, annealed, 99.6+%

GF82635138

Titanium, tube, 500mm, outside diameter 6.35mm, inside diameter 5.53mm, wall thickness 0.41mm, annealed, 99.6+%

GF25880485

Titanium, tube, 100mm, outside diameter 6.35mm, inside diameter 5.53mm, wall thickness 0.41mm, annealed, 99.6+%

GF46592949

Titanium, wire, straight, 1000mm, diameter 1.0mm, as drawn, 99.6+%

GF16602768

Titanium, wire reel, 1m, diameter 0.5mm, as drawn, 99.99+%

267902

Sigma-Aldrich

Titanium, wire, diam. 0.81 mm, 99.7% trace metals basis

348791

Sigma-Aldrich

Titanium, foil, thickness 0.127 mm, 99.7% trace metals basis

GF61322699

Titanium, rod, 100mm, diameter 2mm, annealed, 99.6+%

GF94150593

Titanium, wire reel, 10m, diameter 1.5mm, as drawn, 99.6+%

GF96391402

Titanium, wire reel, 25m, diameter 0.20mm, annealed, 99.6+%

GF76266423

Titanium, wire reel, 0.5m, diameter 1.0mm, annealed, 99.6+%

266035

Sigma-Aldrich

Titanium, wire, diam. 1.0 mm, 99.99% trace metals basis

460400

Sigma-Aldrich

Titanium, wire, diam. 0.25 mm, 99.7% trace metals basis

369489

Sigma-Aldrich

Titanium, foil, thickness 2.0 mm, 99.7% trace metals basis

GF71020235

Titanium, wire reel, 0.5m, diameter 1.0mm, as drawn, 99.6+%

GF90963928

Titanium, rod, 25mm, diameter 50mm, 99.99+%

GF82495198

Titanium, rod, 200mm, diameter 9.5mm, annealed, 99.99+%

GF29391329

Titanium, tube, 1000mm, outside diameter 6.35mm, inside diameter 5.53mm, wall thickness 0.41mm, annealed, 99.6+%

GF68670366

Titanium, tube, 1220mm, outside diameter 2.03mm, inside diameter 1.55mm, wall thickness 0.24mm, annealed, 99.6+%

GF40775610

Titanium, wire reel, 1m, diameter 0.20mm, annealed, 99.6+%

GF05925281

Titanium, microfoil, disks, 10mm, thinness 0.1μm, specific density 42.8μg/cm², permanent mylar 3.5μm support, 99.6+%

GF36425707

Titanium, wire reel, 200m, diameter 0.20mm, annealed, 99.6+%

GF52551222

Titanium, microfoil, disks, 10mm, thinness 0.25μm, specific density 112.6μg/cm², permanent mylar 3.5μm support, 99.6+%

GF50624434

Titanium, wire reel, 200m, diameter 0.25mm, annealed, 99.6+%

GF61228403

Titanium, wire reel, 50m, diameter 0.75mm, as drawn, 99.6+%