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  • Diverse patterns of cell wall mannan/galactomannan occurrence in seeds of the Leguminosae.

Diverse patterns of cell wall mannan/galactomannan occurrence in seeds of the Leguminosae.

Carbohydrate polymers (2012-12-12)
João Francisco Bento, Irineu Mazzaro, Lia Magalhães de Almeida Silva, Renato de Azevedo Moreira, Marília Locatelli Correa Ferreira, Fany Reicher, Carmen Lúcia de Oliveira Petkowicz
ABSTRACT

Endosperms from seeds of different subfamilies of Leguminosae were submitted to sequential aqueous and alkaline aqueous extractions. The extractions from species belonging to the Mimosoideae and Faboideae subfamilies yielded galactomannans with constant Man:Gal ratios, whereas the extractions from Caesalpinioideae seeds gave rise to galactomannans with increasing values of the Man:Gal ratio. The presence of a family of galactomannans within the same species may be a trait found only in Caesalpinioideae subfamily. The final insoluble residues that were obtained after the removal of galactomannans from the Caesalpinioideae and Faboideae subfamilies are composed of pure mannans and do not contain cellulose, while those from the Mimosoideae subfamily are composed of cellulose. A mannan was isolated from the unripe endosperm of Caesalpinia pulcherrima, suggesting no developmental relationship between galactomannan and mannan. These results are consistent with the presence of a distinctive cell wall pattern in the endosperms of Leguminosae species.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
D-Galacto-D-mannan from Ceratonia siliqua, ~95% (HPLC)