58934
SUPELCOSIL™ Suplex pKb-100 (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
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product name
SUPELCOSIL™ Suplex pKb-100 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Agency
suitable for USP L68
feature
endcapped: no
manufacturer/tradename
SUPELCOSIL™
extent of labeling
12.5% carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
25 cm × 4.6 mm
surface area
170 m2/g
surface coverage
surface coverage 3.4 μmol/m2
matrix
silica gel, spherical particle platform
matrix active group
amide, alkyl phase
particle size
5 μm
pore size
120 Å
pH range
2-7.5
application(s)
food and beverages
separation technique
reversed phase
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General description
Suplex pKb-100 columns feature the same bonded phase functionality as SUPELCOSIL LC-ABZ columns. These specially deactivated columns differ in that Suplex pKb-100 is not endcapped, while SUPELCOSIL LC-ABZ is endcapped. The absence of the end-capping reagent results in better performance from Suplex pKb-100 for the strongest basic compounds, while LC-ABZ is preferred when the sample also contains acids and/or zwitterions.
Application
SUPELCOSIL™ Suplex pKb-100 HPLC Column was used in HPLC purification of prepurified fly ovaries. It was also used for HPLC analysis, done for the determination of retinoid.
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
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European journal of biochemistry, 269(14), 3522-3530 (2002-07-24)
Angiotensin converting enzyme (ACE) was already discovered in insects in 1994, but its physiological role is still enigmatic. We have addressed this problem by purifying four new ACE substrates from the ovaries of the grey fleshfly, Neobellieria bullata. Their primary
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