85639

SSC Buffer

for Northern and Southern blotting, powder blend

• Synonym(s): Saline sodium citrate buffer dry blend
• UNSPSC Code: 12161700
• NACRES: NA.25

Properties

• grade: for molecular biology
• product line: BioReagent
• form: powder blend
• impurities: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• solubility: H₂O: 0.263 g/mL, clear, colorless
• storage temp.: room temp

Description

General description

SSC Buffer powder is a standard reagent in Southern and Northern blotting procedures. This powder can be used to make SSC working solutions with concentrations ranging from 0.5x to 20x.

Application

Suitable for:

• Preparing nucleic acids for hybridization
• Hybridization buffer for Northern and Southern blots
• Washing buffer for Northern and Southern blots

Components

• Convenient pack sizes
• Multi-purpose use in hybridizations
• Easy-to-use powder makes fast, inexpensive solutions

SSC Buffer powder contains 0.3 M sodium citrate in 3M NaCl.

Preparation Note

Dissolve 263.5 g in a final volume of 1L molecular biology grade water. The resulting 20x concentrate is approximately pH 7, and can be further diluted with water, as needed.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves