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228842

Sigma-Aldrich

Ethyl Violet

cationic triarylmethane dye

Synonym(s):

Basic violet 4, Ethyl purple 6B

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About This Item

Linear Formula:
C31H42N3Cl
CAS Number:
2390-59-2
Molecular Weight:
492.14
Colour Index Number:
42600
Beilstein:
4117566
EC Number:
219-231-5
MDL number:
MFCD00011765
UNSPSC Code:
12171500
PubChem Substance ID:
24853715
NACRES:
NA.47

Quality Level

200

form

powder

solubility

water: 1 mg/mL

λmax

595 nm

ε (extinction coefficient)

≥12000 at 250-256 nm in water at 0.003 mg/mL
≥17000 at 304-310 nm in water at 0.003 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CCN(CC)c1ccc(cc1)C(\c2ccc(cc2)N(CC)CC)=C3/C=C\C(C=C3)=[N+](\CC)CC

InChI

1S/C31H42N3.ClH/c1-7-32(8-2)28-19-13-25(14-20-28)31(26-15-21-29(22-16-26)33(9-3)10-4)27-17-23-30(24-18-27)34(11-5)12-6;/h13-24H,7-12H2,1-6H3;1H/q+1;/p-1

InChI key

JVICFMRAVNKDOE-UHFFFAOYSA-M

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General description

Ethyl Violet is an olive green colored powder and belongs to the class of triarylmethane dyes. It changes its color from yellow (pH 0.0) to blue (3.5).

Biochem/physiol Actions

Ethyl violet is a cationic triarylmethane dye that is the ethyl homolog of crystal violet. It is a general histological and pathological stain. Ethyl violet has been used to stain pancreatic tissue and elastin, in the detection of gram negative bacteria, and as a reagent for the extractive-spectrophotometric determination of anionic surfactants. Ethyl violet induces mitochondrial permeability. It has been used in the extraction and spectrophotometric determination of copper. Along with the anionic dye zincon, ethyl violet is used in counterion dye of proteins in SDS-polyacrylamide gel electrophoresis.

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H302,H318

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBR9526
0000233367
MKCC4353
MKBV4458V
MKBV1374V

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Ethyl violet. A selective dye for the isolation of gram-negative bacteria.
Litsky, W., et al.
Biotechnic & Histochemistry, 27, 229-232 (1952)
Carla S Oliveira et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 58(13), 2971-2982 (2002-12-13)
We have characterized the spectroscopy properties of crystal violet (CV+) and ethyl violet (EV+) in liquid solutions as a function of the solvent type and dye concentration. The analysis of how solvent properties and dye concentration affects the electronic spectra
Keisuke Morita et al.
Analytical chemistry, 78(22), 7682-7688 (2006-11-16)
A new spectrophotometric method was developed for the determination of low ppb levels of arsenic in water. We found that Ethyl Violet with molybdate-iodine tetrachloride complex forms nanoparticles under acidic conditions, which provide a sensitive probe for molybdoarsenate. The nanoparticles
Jung-Kap Choi et al.
Electrophoresis, 23(24), 4053-4059 (2002-12-14)
A background-free, fast protein staining method in polyacrylamide gel electrophoresis using an acidic dye, zincon (ZC) and a basic dye, ethyl violet (EV) is described. It is based on the counterion dye staining technique that employs two oppositely charged dyes
N Jane Harms et al.
Journal of wildlife diseases, 55(1), 44-53 (2018-06-29)
A central goal for reintroduced populations of threatened wood bison ( Bison bison athabascae) is to maintain them free of diseases of concern, particularly bovine tuberculosis (caused by Mycobacterium bovis) and brucellosis (caused by Brucella abortus). A wood bison population
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