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71003-M

Sigma-Aldrich

Nova Taq DNA Polymerase

Ultrapure recombinant enzyme for dependable PCR amplification

Synonym(s):

DNA Polymerase

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

form

liquid

usage

sufficient for 2000 reactions
sufficient for 400 reactions
sufficient for 80 reactions

feature

dNTPs included: no
hotstart: no

manufacturer/tradename

Novagen®

storage condition

OK to freeze

technique(s)

PCR: suitable

input

purified DNA

storage temp.

−20°C

General description

NovaTaq DNA polymerase is a recombinant form of Thermus aquaticus DNA polymerase. This enzyme is a non-proofreading DNA polymerase. NovaTaq DNA polymerase exhibits 5′-3′ DNA polymerase activity and lacks 3′- 5′ exonuclease activity. The preparation is >95% homogenous by sodium dodecyl sulfate-poly acrylamide gel electrophoresis (SDS-PAGE) and lacks detectable endonuclease activities. It is tested for several quality control assays. This enzyme produces PCR products with 3′-dA overhangs, suitable for cloning with Novagen®Perfectly Blunt®, AccepTor, and LIC Vector Kits. Each item also includes optimized 10X NovaTaq Buffer with 15 mM MgCl2 for routine amplification conditions, plus separate vials of 10X NovaTaq Buffer without MgCl2, and 25 mM MgCl2 to enable convenient optimization of Mg2+ concentration.

Source: Recombinant Thermus aquaticus DNA polymerase expressed in E. coli
Concentration: 5.0 U/μl
Purity: 95% homogeneous by SDS-PAGE
Endonuclease: None detected
Amplification effiency: Functional PCR
Storage: –20°C

Application

NovaTaq DNA polymerase has been used to check the success of the ligation step during cloning. It has also been used in the PCR amplification of cDNA.
NovaTaq DNA Polymerase can be used in standard polymerase chain reaction (PCR) amplification protocols. It is also suitable for colony PCR for screening bacterial colonies.

Features and Benefits

  • Higher yield and PCR specificity
  • Improved low-copy target amplification
  • Amplification of target sequences up to 5 kb
  • Ambient temperature setup compatible with automation
  • Ideal for quantitative and high-throughput applications

Components

•100 U, 500 U, or 5 × 500 UNovaTaq DNA Polymerase

•1 or 2 or 7 × 1.5 ml10X NovaTaq Buffer with MgCl₂

•1 or 2 or 7 × 1.5 ml10X NovaTaq Buffer without MgCl₂

•1 or 2 or 7 × 1.5 ml25 mM MgCl₂

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 74°C, in a reaction containing 25 mM TAPS (tris-[hydroxymethyl]-methyl-amino-propane-sulfonic acid, sodium salt), pH 9.3 at 25°C, 50 mM KCl, 2 mM MgCl₂, 1 mM 2-mercaptoethanol, 0.2 mM dATP, dGTP, and dTTP, 0.1 µM [α-32P]dCTP, and activated salmon sperm DNA.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patents rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Perfectly Blunt is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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