1.05450

GranuCult^® Nutrient agar

ISO 6579, ISO 10273, ISO 19250, ISO 21528, APHA, suitable for microbiology

• UNSPSC Code: 41106200
• NACRES: NA.74

Properties

• Agency: APHA; ISO 10273; ISO 19250; ISO 21528; ISO 6579
• Quality Level: 200
• form: medium granules (dehydrated (DCM))
• manufacturer/tradename: GranuCult^®
• technique(s): microbiological culture: suitable
• pH: 7.0 (37 °C, 20 g/L in H₂O, after autoclaving)
• solubility: 20 g/L
• bulk density: 600 kg/m³
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: heterotrophic bacteria

Description

General description

GranuCult^® Nutrient agar is a universal culture media for cultivating less fastidious microorganisms. The peptone and beef extract provide sufficient nutrients for the growth of non-fastidious bacteria. Both are the sources of nitrogen, vitamins, carbon, and amino acids, while agar is the solidifying agent.

Application

GranuCult^® Nutrient Agar is used for the cultivation of non-fastidious bacteria.

Analysis Note

Appearance (clearness): clear to slightly opalescent
Appearance (colour): yellowish-brown
pH-value (25 °C): 6.8 - 7.2
Solidification behaviour (2 hrs., 45 °C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)):
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)):
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)):
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)):
Inoculum on reference medium (Yersinia enterocolitica ATCC 9610 (WDCM 00038)):
Inoculum on reference medium (Yersinia enterocolitica ATCC 23715 (WDCM 00160)):
Inoculum on reference medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Colony count (Escherichia coli ATCC 8739 (WDCM 00012)):
Colony count (Escherichia coli ATCC 25922 (WDCM 00013)):
Colony count (Salmonella typhimurium ATCC 14028 (WDCM 00031)):
Colony count (Salmonella enteritidis ATCC 13076 (WDCM 00030)):
Colony count (Yersinia enterocolitica ATCC 9610 (WDCM 00038)):
Colony count (Yersinia enterocolitica ATCC 23715 (WDCM 00160)):
Colony count (Staphylococcus aureus ATCC 25923 (WDCM 00034)):
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 70 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 70 %
Recovery on test medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≥ 70 %
Recovery on test medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≥ 70 %
Recovery on test medium (Yersinia enterocolitica ATCC 9610 (WDCM 00038)): ≥ 70 %
Recovery on test medium (Yersinia enterocolitica ATCC 23715 (WDCM 00160)): ≥ 70 %
Recovery (Staphylococcus aureus ATCC 25923 (WDCM00034)): ≥ 70 %
Incubation:
24 ± 2 at 37 ± 1 °C aerobic
Yersinia 24 ± 2 hours at 30 ± 1 °C aerobic
A recovery rate of 70 % is equivalent to a productivity value of 0.7.
The indicated colony counts result from the sum of a triple determination.
Reference media: Tryptic Soy Agar

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3