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  • Simultaneous determination of five 1,4-dihydropyridines in pharmaceutical formulations by high-performance liquid chromatography-amperometric detection.

Simultaneous determination of five 1,4-dihydropyridines in pharmaceutical formulations by high-performance liquid chromatography-amperometric detection.

Journal of chromatography. A (2004-04-03)
Ana B Baranda, Rosa M Jiménez, Rosa M Alonso
ABSTRACT

A high-performance liquid chromatographic method with electrochemical detection was developed for the simultaneous determination of five 1,4-dihydropyridines: amlodipine, nitrendipine, felodipine, lacidipine and lercanidipine. These drugs are widely used in the treatment of hypertension, angina pectoris and the therapy of cerebrovascular spasms of various origins. The chromatographic separation was performed on a Supelcosil LC ABZ + Plus C18 column with a mobile phase consisting of acetonitrile-10 mM acetate buffer (72:28, v/v) at a flow rate of 1 ml/min. The temperature was set at 30 +/- 0.2 degrees C. The amperometric detector, equipped with a glassy carbon electrode was operated at +1100 mV versus Ag/AgCl in the direct current mode. Under these chromatographic conditions, the drugs eluted in less than 12 min. The method showed to be linear over the range 4.5-15 microg/ml with a within-day and day-to-day repeatabilities in terms of R.S.D. lower than 15%, an accuracy greater than 98% and detection limits varying from 90 ng/ml (amlodipine) to 1.55 microg/ml (nitrendipine). The method was successfully applied to commercially available pharmaceuticals with relative errors lower than 5%. The validity of the method was examined comparing the results obtained with those of HPLC with photometric detection.

MATERIALS
Product Number
Brand
Product Description

Supelco
SUPELCOSIL LC-ABZ Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 2.1 mm
Supelco
SUPELCOSIL LC-ABZ Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 2.1 mm
Supelco
SUPELCOSIL LC-ABZ Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
SUPELCOSIL LC-ABZ HPLC Column, 5 μm particle size, L × I.D. 25 cm × 3 mm
Supelco
SUPELCOSIL LC-ABZ HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm
Supelco
SUPELCOSIL LC-ABZ HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
SUPELCOSIL LC-ABZ HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
Supelco
SUPELCOSIL LC-ABZ Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
SUPELCOSIL LC-ABZ HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
SUPELCOSIL LC-NH2-NP Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
SUPELCOSIL LC-NH2-NP HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm