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  • Argentation high performance liquid chromatography on-line coupled to gas chromatography for the analysis of monounsaturated polyolefin oligomers in packaging materials and foods.

Argentation high performance liquid chromatography on-line coupled to gas chromatography for the analysis of monounsaturated polyolefin oligomers in packaging materials and foods.

Journal of chromatography. A (2015-06-04)
Martin Lommatzsch, Maurus Biedermann, Thomas J Simat, Koni Grob
ABSTRACT

Multidimensional chromatography based on two-dimensional high performance liquid chromatography on-line coupled to gas chromatography (on-line HPLC-HPLC-GC) enables the separate analysis of saturated, monounsaturated and aromatic hydrocarbons in packaging materials like polyolefins or paperboard and their migrates into foods. Since normal-phase HPLC on silica gel did not preseparate saturated from monounsaturated hydrocarbons, a separation step on a normal-phase HPLC column treated in the laboratory with an optimized amount of silver nitrate was added. The preparation of this HPLC column and the instrumental set-up are described, followed by examples showing the potential of the method. In a preliminary investigation of 11 polyolefin granulates for food contact up to 40% monounsaturated hydrocarbons among the oligomers C16-35 were determined.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1-Dodecene, ≥99.0% (GC)
Sigma-Aldrich
2-Methyl-1-undecene, 97%
Sigma-Aldrich
1-Dodecene, 95%
Sigma-Aldrich
Rhein, technical grade
Sigma-Aldrich
Rhein
Sigma-Aldrich
TBB, ≥98% (HPLC), solid
Sigma-Aldrich
Perylene, ≥99%
Sigma-Aldrich
Perylene, sublimed grade, ≥99.5%
Sigma-Aldrich
Benzene, anhydrous, 99.8%
Sigma-Aldrich
Benzene, JIS special grade, ≥99.5%
Sigma-Aldrich
Benzene, JIS 300, ≥99.5%, for residue analysis
Sigma-Aldrich
Benzene, SAJ first grade, ≥99.0%