- Generation of a Sprague-Dawley-GFP rat iPS cell line.
Generation of a Sprague-Dawley-GFP rat iPS cell line.
Stem cell research (2017-07-06)
Giulia Coppiello, Gloria Abizanda, Natalia Aguado, Elena Iglesias, Olalla Iglesias-Garcia, Antonio Lo Nigro, Felipe Prosper, Xabier L Aranguren
PMID28677538
ABSTRACT
We generated a rat iPSC line called ATCi-rSD95 from transgenic Sprague-Dawley GFP fetal fibroblasts. Established ATCi-rSD95 cells present a normal karyotype, silencing of the transgenes and express pluripotency-associated markers. Additionally, ATCi-rSD95 cells are able to form teratoma with differentiated cells derived from the three germ-layers that maintain the GFP expression.