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  • Decellularized Human Chorion Membrane as a Novel Biomaterial for Tissue Regeneration.

Decellularized Human Chorion Membrane as a Novel Biomaterial for Tissue Regeneration.

Biomolecules (2020-08-23)
Laura P Frazão, Joana Vieira de Castro, Cristina Nogueira-Silva, Nuno M Neves
ABSTRACT

Although some placenta-derived products are already used for tissue regeneration, the human chorion membrane (HCM) alone has been poorly explored. In fact, just one study uses decellularized HCM (dHCM) with native tissue architecture (i.e., without extracellular matrix (ECM) suspension creation) as a substrate for cell differentiation. The aim of this work is to fully characterize the dHCM for the presence and distribution of cell nuclei, DNA and ECM components. Moreover, mechanical properties, in vitro biological performance and in vivo biocompatibility were also studied. Our results demonstrated that the HCM was successfully decellularized and the main ECM proteins were preserved. The dHCM has two different surfaces, the reticular layer side and the trophoblast side; and is biocompatible both in vitro and in vivo. Importantly, the in vivo experiments demonstrated that on day 28 the dHCM starts to be integrated by the host tissue. Altogether, these results support the hypothesis that dHCM may be used as a biomaterial for different tissue regeneration strategies, particularly when a membrane is needed to separate tissues, organs or other biologic compartments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Papain from papaya latex, lyophilized powder, ≥10 units/mg protein
Sigma-Aldrich
Laminin from human placenta, liquid, BioReagent, suitable for cell culture
Sigma-Aldrich
Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
Sigma-Aldrich
Collagen from human placenta, Bornstein and Traub Type IV, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Monoclonal Anti-Laminin antibody produced in mouse, clone LAM-89, ascites fluid
CellCrown inserts, 24 well plate inserts, non-sterile