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  • The Ski protein can inhibit ligand induced RARalpha and HDAC3 degradation in the retinoic acid signaling pathway.

The Ski protein can inhibit ligand induced RARalpha and HDAC3 degradation in the retinoic acid signaling pathway.

Biochemical and biophysical research communications (2009-04-04)
Hong-Ling Zhao, Nobuhide Ueki, Katherine Marcelain, Michael J Hayman
ABSTRACT

Recent data has implicated the Ski protein as being a physiologically relevant negative regulator of signaling by retinoic acid (RA). The mechanism by which Ski represses RA signaling is unknown. Co-immunoprecipitation and immunofluorescence assay showed that Ski and RARalpha are in the same complex in both the absence and presence of RA, which makes Ski different from other corepressors. We determined that Ski can stabilize RARalpha and HDAC3. These results suggest that Ski represses RA signaling by stabilizing corepressor complex.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-HDAC3 Antibody, clone 3G6, clone 3G6, Upstate®, from mouse