P1944

Phenol:Chloroform:Iso-amyl alcohol (125:24:1)

for molecular biology

• Synonym(s): phenol chloroform RNA extraction
• UNSPSC Code: 12190000
• NACRES: NA.52

Properties

• grade: for molecular biology
• Assay: ≥99.0%
• form: liquid
• pH: 4.3-4.7
• application(s): life science and biopharma
• storage temp.: 2-8°C

Description

General description

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.

Application

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:

• for the extraction and purification of RNA.
• to isolate RNA from Arabidopsis siliques.
• to extract proteins and DNA from polysomal and non-polysomal fractions.
• in TRIzol methods to prepare and extract miRNAs from cells, tissue, and whole flies.

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H301 + H311 + H331,H314,H341,H351,H361d,H372,H373,H411
• Precautionary Statements: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2
• Target Organs: Liver,Kidney, Nervous system,Kidney,Liver,Skin
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): 175.0 °F - closed cup
• Flash Point(C): 79.44 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves