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08714

Millipore

Methyl Red solution

suitable for microbiology

Synonym(s):

Methyl red indicator solution

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About This Item

Empirical Formula (Hill Notation):
C15H15N3O2
CAS Number:
493-52-7
Molecular Weight:
269.30
Beilstein:
1843037
MDL number:
MFCD00002425
UNSPSC Code:
41171621
PubChem Substance ID:
329748627
NACRES:
NA.85

Agency

according to GB 4789.30-2016
according to ISO 22964:2017

Quality Level

200

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

composition

dist. water, 200 mL
ethanol 95%, 300 mL
methyl red, 0.1 g

technique(s)

microbe id | metabolite detection: suitable

application(s)

clinical testing
environmental
food and beverages
microbiology

suitability

Enterococcus spp.
Escherichia coli
Klebsiella spp.
Proteus spp.
Pseudomonas spp.
Staphylococcus spp.
bacteria

SMILES string

CN(C)c1ccc(cc1)\N=N\c2ccccc2C(O)=O

InChI

1S/C15H15N3O2/c1-18(2)12-9-7-11(8-10-12)16-17-14-6-4-3-5-13(14)15(19)20/h3-10H,1-2H3,(H,19,20)/b17-16+

InChI key

CEQFOVLGLXCDCX-WUKNDPDISA-N

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General description

Methyl red solution is an azo dye which turns to red when pH is below 4.4 (yellow pH < 6.2, orange pH 4.4-6.2). Some bacteria utilize glucose to form large amounts of acid with the result that the pH value of the medium falls distinct. Other species produce no or less free acid. This difference can be visualized by using methyl red. This test is used to differentiate enteric bacteria.

Application

Methyl red solution is a pH indicator dye recommended for detection of bacteria producing acid from glucose fermentation such as Enterobacteriaceae in various samples.

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

69.8 °F

Flash Point(C)

21 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL8848
BCCL0746
BCCK1481
BCCJ3868
BCCG7250

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S S Gomare et al.
Journal of applied microbiology, 106(3), 993-1004 (2009-02-04)
To evaluate the potential of Brevibacillus laterosporus MTCC 2298 for the decolourization of different textile azo dyes including methyl red, mechanism of biotransformation and the toxicity of products. Brevibacillus laterosporus showed decolourization of thirteen different azo dyes including methyl red.
Danmeng Shuai et al.
Environmental science & technology, 44(5), 1773-1779 (2010-02-11)
Azo dyes are widespread pollutants and potential cocontaminants for nitrate; we evaluated their effect on catalytic reduction of a suite of oxyanions, diatrizoate, and N-nitrosodimethylamine (NDMA). The azo dye methyl orange significantly enhanced (less than or equal to a factor
Sofia Babanova et al.
Journal of bioscience and bioengineering, 112(4), 379-387 (2011-07-26)
Soluble artificial mediators are often applied to enhance the electron transfer from living cells to an anode in microbial fuel cells. Recently, we have demonstrated that the Candida melibiosica 2491 yeast strain possesses electrogenic properties and can be used as
Angel Martinez et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(52), 20891-20896 (2011-12-14)
Noncontact optical trapping and manipulation of micrometer- and nanometer-sized particles are typically achieved by use of forces and torques exerted by tightly focused high-intensity laser beams. Although they were instrumental for many scientific breakthroughs, these approaches find few technological applications
Taiga Fujii et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 18(35), 10865-10872 (2012-07-26)
Asymmetric dye clusters with a single fluorophore (Cy3) and multiple quenchers (4'-methylthioazobenzene-4-carboxylate, methyl red, and 4'-dimethylamino-2-nitroazobenzene-4-carboxylate) were prepared. The dye and one-to-five quenchers were tethered through D-threoninol to opposite strands of a DNA duplex. NMR analysis revealed that the clusters
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