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69820-M

Sigma-Aldrich

pT7Blue T-Vector - Novagen

designed for simplified cloning of PCR products

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About This Item

UNSPSC Code:
41106610
NACRES:
NA.51

Quality Level

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

storage temp.

−20°C

General description

The pT7Blue T-Vector is designed for simplified cloning of PCR products. Many thermostable DNA polymerases, such as Taq DNA polymerase, leave single 3′ A-nucleotide overhangs on their reaction products. These molecules can be ligated directly to a vector containing compatible single T-nucleotide overhangs. The pT7Blue vector has been specifically constructed for this application and has been prepared for T-cloning by digestion with EcoRV followed by the addition of single 3′ dT residues at each end. The T-Vector is provided ready to ligate with DNA amplified with any DNA polymerase that leaves single 3′ dA overhangs. Target inserts having other types of ends, such as reaction products of proofreading polymerase or restriction fragments, can be easily prepared for T-cloning with the Single dA<TMSYMBOL></TMSYMBOL> Tailing Kit. pT7Blue contains the pUC19 backbone (including high-copy number origin or replication and lac sequences), a T7 promoter, f1 origin of replication, and a modified multiple cloning region.

Components

  • 2g pT7Blue T-Vector, 50 ng/l
  • 25 ng T-Vector Positive Control Insert, 2.5 ng/l

Warning

Toxicity: Standard Handling (A)

Legal Information

This product is only available in Japan.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PT7BLUE is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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