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Key Documents

G001585

HPLC Analysis of Angiotensins on Discovery® BIO Wide Pore C18

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Discovery BIO Wide Pore C18, 15 cm × 4.6 mm I.D., 5 μm particles (568222-U)
mobile phase: [A] (10 mM NH4OAc, pH 7):(50% CH3CN in 20 mM NH4OAc, pH 7), 65:35 ; [B] (10 mM NH4OAc, pH 7):(50% CH3CN in 20 mM NH4OAc, pH 7), 25:75
gradient: 0-100% B in 12.5 min
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 254 nm
injection: 6 μL (10 μg each) in water

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Angiotensin III trifluoroacetate salt hydrate

analytical column

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