"Cluster-ion" bombardment of biological macromolecules: Morphological evidence for disassembly of single tobacco mosaic virus particles.

"Cluster-ion" bombardment of biological macromolecules: Morphological evidence for disassembly of single tobacco mosaic virus particles.

Proceedings of the National Academy of Sciences of the United States of America (1987-01-01)

M C Ledbetter, R J Beuhler, L Friedman

ABSTRACT

Cluster ions containing 50, 100, or 200 water molecules and one proton are accelerated to over 275 kV and impacted on thin carbon films on which tobacco mosaic virus has been dispersed. After bombardment the films are examined with a transmission electron microscope to study induced morphological alterations with respect to the energy transfer processes that take place during and after single cluster-ion impact. Observations on tobacco mosaic virus indicate that a single cluster impact results in the removal of a cylindrical segment of the virus. No fragments from the excised TMV segment were observed on the carbon foil. We speculate that cluster-ion bombardment may become a useful analytical technique for structural studies of large biological macromolecular complexes.

MATERIALS

Product Number

Brand

Product Description

GF26559513

Carbon, foil, 150x150mm, thickness 5.0mm, rigid graphite, medium grain size, 99.5%

GF27136100

Carbon, foil, 25x25mm, thickness 0.25mm, rigid graphite, fine grain size, 99.997%

GF29446814

Carbon, foil, 500x500mm, thickness 0.5mm, flexible graphite, 99.8%

GF08538970

Carbon, foil, 250x250mm, thickness 0.35mm, flexible graphite, 99.8%

GF34902109

Carbon, foil, 10x10mm, thickness 2.0mm, hOpg

GF62540232

Carbon, foil, 25x25mm, thickness 0.25mm, rigid graphite, fine grain size, 99.95%

GF15163297

Carbon, foil, 12.5x12.5mm, thickness 0.125mm, rigid graphite, fine grain size, 99.95%

GF87162857

Carbon, foil, 50x50mm, thickness 0.5mm, flexible graphite, 99.8%

GF85706400

Carbon, foil, 50x50mm, thickness 0.125mm, rigid graphite, fine grain size, 99.95%

GF97410708

Carbon, foil, 250x250mm, thickness 1.0mm, flexible graphite, 99.8%

GF95868365

Carbon, foil, 150x150mm, thickness 0.125mm, rigid graphite, fine grain size, 99.95%

GF83177949

Carbon, foil, 12mm disks, thickness 0.5mm, rigid graphite, fine grain size, 99.95%

GF37688754

Carbon, foil, 25x25mm, thickness 0.5mm, rigid graphite, fine grain size, 99.997%

GF31912039

Carbon, foil, 150x150mm, thickness 0.5mm, rigid graphite, fine grain size, 99.95%

GF99536338

Carbon, foil, 500x500mm, thickness 0.2mm, flexible graphite, 99.8%

GF09288906

Carbon, foil, 15x15mm, thickness 2.0mm, hOpg

GF36978104

Carbon, foil, 150x150mm, thickness 0.25mm, rigid graphite, fine grain size, 99.95%

GF39666077

Carbon, foil, 25x25mm, thickness 0.2mm, flexible graphite, 99.8%

GF29579787

Carbon, foil, 200x200mm, thickness 1.0mm, rigid graphite, fine grain size, 99.95%

GF04191971

Carbon, foil, 150x150mm, thickness 0.2mm, flexible graphite, 99.8%

GF16618327

Carbon, foil, 15mm disks, thickness 0.2mm, flexible graphite, 99.8%

GF64201249

Carbon, foil, 25x25mm, thickness 2.5mm, rigid graphite, fine grain size, 99.95%

GF13787332

Carbon, foil, 50x50mm, thickness 1.0mm, flexible graphite, 99.8%

GF00462912

Carbon, foil, 25x25mm, thickness 0.1mm, pyrolytic graphite, 99.99%

GF77290162

Carbon, foil, 25mm disks, thickness 0.2mm, flexible graphite, 99.8%

GF13406633

Carbon, foil, 250x250mm, thickness 0.5mm, flexible graphite, 99.8%

GF37642822

Carbon, foil, 500x500mm, thickness 0.35mm, flexible graphite, 99.8%

GF02861782

Carbon, foil, not light tested, 300x300mm, thickness 0.075mm, flexible graphite, 99.8%

GF01564017

Carbon, foil, 25x25mm, thickness 0.5mm, rigid graphite, fine grain size, 99.95%

GF78361285

Carbon, foil, 150x150mm, thickness 0.5mm, flexible graphite, 99.8%