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T3405

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine dihydrochloride

chromogenic, tablet

Synonym(s):

4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride

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About This Item

Empirical Formula (Hill Notation):
C16H20N2 · 2HCl
CAS Number:
64285-73-0
Molecular Weight:
313.27
EC Number:
264-769-6
MDL number:
MFCD00012961
UNSPSC Code:
12352204
PubChem Substance ID:
24900140
NACRES:
NA.83

product name

3,3′,5,5′-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet

form

tablet

mp

≥300 °C

storage temp.

2-8°C

SMILES string

Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H

InChI key

NYNRGZULARUZCC-UHFFFAOYSA-N

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Application

3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for the
  • anti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice sera
  • anti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysate
  • in enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Darcy S O Mora et al.
Fish & shellfish immunology, 71, 255-263 (2017-09-04)
An experimental contraceptive vaccine was evaluated in Atlantic salmon (Salmo salar). A peptide derived from the beta subunit of luteinizing hormone (LH) was conjugated to two different carrier proteins, bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH), and formulated
A novel assay for the evaluation of the prooxidant-antioxidant balance, before and after antioxidant vitamin administration in type II diabetes patients
Alamdari DH, et al.
Clinical Biochemistry, 40(3-4), 248-254 (2007)
C Flohr et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 40(1), 131-142 (2009-09-18)
Observational evidence suggests that infection with helminths protects against allergic disease and allergen skin sensitization. It is postulated that such effects are mediated by helminth-induced cytokine responses, in particular IL-10. We tested this hypothesis in a rural area of central
E Rosenqvist et al.
Methods in molecular medicine, 66, 255-273 (2001-01-01)
Enzyme-linked immunosorbent assay (ELISA, EIA) is a highly versatile and sensitive technique that can be used for quantitative as well as qualitative determination of almost any antigen or antibody. Reagents are stable, non-radioactive and, in most cases, commercially available. Owing
Mengyao Wang et al.
PloS one, 13(1), e0190452-e0190452 (2018-01-06)
The impact of epidemic Staphylococcus aureus (S. aureus) on public health is increasing. Because of the abuse of antibiotics, the antibiotic resistance of S. aureus is increasing. Thus, there is an urgent need to develop new immunotherapies and immunoprophylaxes. Previous
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