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P7828

Sigma-Aldrich

Percoll®

suitable for plant cell culture

Synonym(s):

density gradient medium

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About This Item

MDL number:
MFCD00131830
UNSPSC Code:
10171502
NACRES:
NA.72

sterility

aseptically filled

Quality Level

200

technique(s)

cell culture | plant: suitable

pH

8.9 (20 °C)

application(s)

agriculture

storage temp.

2-8°C

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Application

Percoll consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water) which have been coated with polyvinylpyrrolidone (PVP). It is used to establish non-toxic low viscosity, low osmolarity density gradients that can be used to isolate cells, organelles and viruses. It has been used to separate inner and outer membrane vesicles from E. coli. Percoll is isosmotic throughout the gradient, non-toxic, and is easily removed from the purified materials.
Percoll is used in plant research for protoplast and organelle (chloroplast, mitochondria) separation.
Percoll® has been used in the density gradient centrifugation isolation of intact chloroplasts from green alga, decidual stromal cells and distinct populations of rooster sperm.

Legal Information

Percoll is a registered trademark of Cytiva

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Guangkun Yin et al.
Plant & cell physiology, 50(7), 1305-1318 (2009-06-13)
The development of mitochondria during seed germination is essential for plant growth. However, the developmental process is still poorly understood. Temperature plays a key role in soybean germination, and in this study we characterized the mitochondrial ultrastructure and proteome after
A rapid method for chloroplast isolation from the green alga Chlamydomonas reinhardtii
Mason CB, et al.
Nature Protocols, 1(5), 2227-2227 (2006)
Xiang-Jie Yin et al.
Clinical proteomics, 18(1), 9-9 (2021-02-24)
Pregnancy is a complicated physiological process. The multifaceted regulation of maternal-fetal interface is of great importance for maintaining normal pregnancy and avoiding fetal rejection and secondary abortion. Previous studies have focused on the clinical features or pathological biomarkers of fetal
Sperm Collection of Differential Quality Using Density Gradient Centrifugation
Ahammad MU, et al.
Journal of Visualized Experiments, 145(141), e58833-e58833 (2018)
Teresa D'Amico Oblak et al.
The Analyst, 134(1), 188-193 (2008-12-17)
A simple poly(dimethylsiloxane) (PDMS) microchip was employed to establish a relationship between red blood cell (RBC) antioxidant status and the ability of RBCs to interact with metal-activated C-peptide, a bio-active peptide reported to reduce some complications often associated with diabetes.
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Protocols

How to Make and Use Percoll Gradients

To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

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