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12013819001

Roche

Anti-HA-Peroxidase, High Affinity

from rat IgG1

Synonym(s):

antibody

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About This Item

UNSPSC Code:
12352203

biological source

rat

Quality Level

100

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

clone 3F10, monoclonal

form

lyophilized (clear, colorless solution after reconstitution)

packaging

pkg of 25 U (25 μg)

manufacturer/tradename

Roche

isotype

IgG1

epitope sequence

YPYDVPDYA

storage temp.

2-8°C

Related Categories

General description

Anti-HA-Peroxidase, High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10), conjugated to peroxidase.

Specificity

Anti-HA-Peroxidase, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein.
This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).

Immunogen

The epitope consists of amino acids 98-106 from the human influenza virus hemagglutinin protein.

Application

  • Use Anti-HA-Peroxidase, High Affinity for the detection of HA-tagged recombinant proteins using: ELISA
  • Western blot
Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling.

Preparation Note

Stabilizers: proteinaceous stabilizers
Working concentration: The working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
  • Dot blot: 50 mU/ml
  • ELISA: 25 mU/ml
  • Western blot: 50 mU/ml

Reconstitution

Add 1.0 ml double-distilled water for a final concentration of 25 U/mL.
Rehydrate for 10 min prior to use.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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