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Key Documents

AP510P

Sigma-Aldrich

Sheep Anti-Rabbit IgG Antibody, HRP conjugate

1 mg/mL, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

sheep

Quality Level

conjugate

peroxidase conjugate

antibody form

purified antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

rabbit

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

General description

Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules. This antibody is processed from Cat. No. AP510, Sheep anti-Rabbit IgG.

Specificity

This antibody reacts with rabbit IgG and rabbit IgG (Fc). There may be minimal cross-reactivity against Human IgG and Rabbit IgM, with minimal to no cross-reactivity against Mouse IgG, Rat IgG, Chicken IgY, Guinea Pig IgG, and Hamster IgG.

Application

Sheep Anti-Rabbit IgG Antibody, HRP conjugate validated for use in western blotting.

Quality

Evaluated by Western Blot in Rabbit IgG recombinant protein.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Rabbit IgG in 0.5 µg of Rabbit IgG recombinant protein.

Target description

~53 kDa observed

Physical form

Purified sheep polyclonal in buffer containing PBS, StabilZyme® HRP Conjugate Stabilizer, and 0.05% ProClin 150.

Analysis Note

Control
Rabbit IgG recombinant protein

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
StabilZyme is a registered trademark of SurModics, Inc.

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Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Repr. 1A

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jie Zhou et al.
Cell reports, 38(6), 110344-110344 (2022-01-31)
SARS-CoV-2 has a broad mammalian species tropism infecting humans, cats, dogs, and farmed mink. Since the start of the 2019 pandemic, several reverse zoonotic outbreaks of SARS-CoV-2 have occurred in mink, one of which reinfected humans and caused a cluster
Ryan J Bloom et al.
Nature methods, 11(11), 1147-1153 (2014-09-15)
Synthetic genetic circuits incorporating regulatory components based on RNA interference (RNAi) have been used in a variety of systems. A comprehensive understanding of the parameters that determine the relationship between microRNA (miRNA) and target expression levels is lacking. We describe
Ecco Staller et al.
The Journal of general virology, 102(9) (2021-09-16)
Viruses require host factors to support their replication, and genetic variation in such factors can affect susceptibility to infectious disease. Influenza virus replication in human cells relies on ANP32 proteins, which are involved in assembly of replication-competent dimeric influenza virus
Yurika Matsui et al.
DNA and cell biology, 38(1), 91-106 (2018-11-22)
The considerable amount of experimental evidence has defined the Hippo pathway as a tumor suppressive pathway and increased expression and/or activity of its oncogenic effectors is frequently observed in cancer. However, clinical studies have failed to attribute cancer development and
Bhakti Mistry et al.
Journal of virology, 94(3) (2019-11-07)
The avian-origin influenza A virus polymerase is restricted in human cells. This restriction has been associated with species differences in host factor ANP32A. Avian ANP32A supports the activity of an avian-origin polymerase. However, the avian-origin polymerase is incompatible with human

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