C85778

o-Cresolphthalein

Indicator grade, powder

• Empirical Formula (Hill Notation): C₂₂H₁₈O₄
• CAS Number: 596-27-0
• Molecular Weight: 346.38
• Beilstein: 310554
• EC Number: 209-881-8
• MDL number: MFCD00005912
• UNSPSC Code: 12171500
• PubChem Substance ID: 24893078
• NACRES: NA.47

Properties

• product name: o-Cresolphthalein, indicator grade
• grade: indicator grade
• form: powder
• technique(s): titration: suitable
• color: beige
• pH: 8.2-9.8, colorless to red
• mp: 219-221 °C (lit.)
• λmax: 381 nm (2nd); 566 nm
• ε (extinction coefficient): ≥10000 at 294-300 nm; ≥39000 at 564-570 nm; ≥5000 at 378-384 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cc1cc(ccc1O)C2(OC(=O)c3ccccc23)c4ccc(O)c(C)c4
• InChI: 1S/C22H18O4/c1-13-11-15(7-9-19(13)23)22(16-8-10-20(24)14(2)12-16)18-6-4-3-5-17(18)21(25)26-22/h3-12,23-24H,1-2H3
• InChI key: CPBJMKMKNCRKQB-UHFFFAOYSA-N

Description

Application

O-Cresolphthalein has been used as a component in calcifying media for calcification assay.

Biochem/physiol Actions

o-Cresolphthalein is a reversible pH indicator. The kinetics of the reverse pathway (red-purple to colorless) is slow, thereby making it an appropriate molecule to check flow patterns in ducts with corrugated walls. It is also used for the colorimetric determination of calcium content via the o-cresolphthalein complexone method. Binding of calcium to o-cresolphthalein, in the presence of alkaline conditions (pH 10-12), results in the formation of a red complex with an absorbance at 570-575nm.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves