Skip to Content
Merck

Pegylation increases platelet biocompatibility of gold nanoparticles.

Journal of biomedical nanotechnology (2014-04-23)
Maria Jose Santos-Martinez, Kamil Rahme, J Jose Corbalan, Colm Faulkner, Justin D Holmes, Lidia Tajber, Carlos Medina, Marek Witold Radomski
ABSTRACT

The increasing use of gold nanoparticles in medical diagnosis and treatment has raised the concern over their blood compatibility. The interactions of nanoparticles with blood components may lead to platelet aggregation and endothelial dysfunction. Therefore, medical applications of gold nanoparticles call for increased nanoparticle stability and biocompatibility. Functionalisation of nanoparticles with polythelene glycol (PEGylation) is known to modulate cell-particle interactions. Therefore, the aim of the current study was to investigate the effects of PEGylated-gold nanoparticles on human platelet function and endothelial cells in vitro. Gold nanoparticles, 15 nm in diameter, were synthesised in water using sodium citrate as a reducing and stabilising agent. Functionalised polyethylene glycol-based thiol polymers were used to coat and stabilise pre-synthesised gold nanoparticles. The interaction of gold nanoparticles-citrate and PEGylated-gold nanoparticles with human platelets was measured by Quartz Crystal Microbalance with Dissipation. Platelet-nanoparticles interaction was imaged using phase-contrast, scanning and transmission electron microscopy. The inflammatory effects of gold nanoparticles-citrate and PEGylated-gold nanoparticles in endothelial cells were measured by quantitative real time polymerase chain reaction. PEGylated-gold nanoparticles were stable under physiological conditions and PEGylated-gold nanoparticles-5400 and PEGylated-gold nanoparticles-10800 did not affect platelet aggregation as measured by Quartz Crystal Microbalance with Dissipation. In addition, PEGylated-gold nanoparticles did not induce an inflammatory response when incubated with endothelial cells. Therefore, this study shows that PEGylated-gold nanoparticles with a higher molecular weight of the polymer chain are both platelet- and endothelium-compatible making them attractive candidates for biomedical applications.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Gold, foil, thickness 0.025 mm, 99.99% trace metals basis
Sigma-Aldrich
Gold, evaporation slug, diam. × L 0.3 cm × 0.6 cm, 99.99% trace metals basis
Sigma-Aldrich
Gold, nanopowder, <100 nm particle size, 99.9% trace metals basis
Sigma-Aldrich
Gold coated glass slide, 99.999% (Au), layer thickness 100 Å
Sigma-Aldrich
Gold coated glass slide, 99.999% (Au), layer thickness 1000 Å
Sigma-Aldrich
Gold, powder, <850 μm, ≥99.99% trace metals basis
Sigma-Aldrich
Gold, wire, diam. 0.5 mm, 99.999% trace metals basis
Sigma-Aldrich
Gold, wire, diam. 1.0 mm, 99.99% trace metals basis
Sigma-Aldrich
Gold, wire, diam. 0.25 mm, 99.99% trace metals basis
Sigma-Aldrich
Gold, foil, thickness 0.1 mm, 99.99% trace metals basis
Sigma-Aldrich
Gold, foil, thickness 0.127 mm, 99.99% trace metals basis
Sigma-Aldrich
Gold coated glass cover slip, 99.999% (Au), layer thickness 100 Å, L × W × thickness 22 mm × 22 mm × 130-170 μm, square
Sigma-Aldrich
Gold coated glass cover slip, 99.999% (Au), diam. × thickness 15 mm × 130-170 μm, layer thickness 100 Å
Gold, tube, 50mm, outside diameter 3.0mm, inside diameter 2.8mm, wall thickness 0.10mm, as drawn, 99.95%
Gold, tube, 50mm, outside diameter 1.60mm, inside diameter 0.6mm, wall thickness 0.5mm, as drawn, 99.95%
Gold, rod, 100mm, diameter 1.6mm, as drawn, 99.95%
Gold, microleaf disks, 10mm, thinness 0.25μm, specific density 483μg/cm2, removable support, 99.99+%
Gold, insulated wire, 2m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyester insulation, 99.99%
Gold, rod, 25mm, diameter 2.0mm, as drawn, 99.95%
Gold, tube, 200mm, outside diameter 1.60mm, inside diameter 0.6mm, wall thickness 0.5mm, as drawn, 99.95%
Gold, tube, 50mm, outside diameter 2.0mm, inside diameter 1.7mm, wall thickness 0.15mm, as drawn, 99.95%
Gold, tube, 100mm, outside diameter 2.0mm, inside diameter 1.7mm, wall thickness 0.15mm, as drawn, 99.95%
Gold, insulated wire, 2m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%
Gold, insulated wire, 5m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%
Gold, insulated wire, 1m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%
Gold, tube, 100mm, outside diameter 2.0mm, inside diameter 1.8mm, wall thickness 0.1mm, as drawn, 99.95%
Gold, tube, 50mm, outside diameter 2.0mm, inside diameter 1.8mm, wall thickness 0.1mm, as drawn, 99.95%
Gold, microleaf disks, 10mm, thinness 1.0μm, specific density 1932μg/cm2, removable support, 99.99+%
Gold, insulated wire, 0.5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%
Gold, insulated wire, 5m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyester insulation, 99.99%