MCKP1-110K-PBK28
MILLIPLEX® Mouse Immuno-Oncology Checkpoint Protein Panel 1
Immuno-Oncology Multiplex Assay
About This Item
Quality Level
species reactivity
mouse
manufacturer/tradename
Milliplex®
assay range
accuracy: 101
(Galectin-1)
accuracy: 103
(CD80/B7-1)
accuracy: 103
(IFNγ)
accuracy: 104
(CTLA-4/CD152)
accuracy: 104
(PD-L2)
accuracy: 104
(TLR-2)
accuracy: 105
(4-1BBL/TNFSF9)
accuracy: 105
(BCA-1/CXCL-13)
accuracy: 105
(CD25/IL-2Rα)
accuracy: 105
(IL-10)
accuracy: 105
(LAG-3)
accuracy: 106
(5’-NT/CD73)
accuracy: 106
(PD-1)
accuracy: 106
(TIM-3)
accuracy: 106
(TNFα)
accuracy: 107
(CD137/4-1BB)
accuracy: 107
(CD27)
accuracy: 108
(E-Cadherin)
accuracy: 108
(Galectin-3)
accuracy: 108
(HVEM)
accuracy: 109
(CD40)
accuracy: 109
(GITR)
accuracy: 110
(B7-H2/ICOSL)
accuracy: 110
(CD226/DNAM-1)
accuracy: 111
(BTLA)
accuracy: 94
(GranzymeB)
accuracy: 95
(B7-H3/CD276)
accuracy: 96
(PD-L1)
standard curve range: 1.4 - 1000 pg/mL
(HVEM)
standard curve range: 11 - 8000 pg/mL
(IL-10)
standard curve range: 137 - 100000 pg/mL
(5’-NT/CD73)
standard curve range: 14 - 10000 pg/mL
(4-1BBL/TNFSF9)
standard curve range: 14 - 10000 pg/mL
(CD226/DNAM-1)
standard curve range: 14 - 10000 pg/mL
(CD40)
standard curve range: 14 - 10000 pg/mL
(PD-L1)
standard curve range: 2.1 - 1500 pg/mL
(IFNγ)
standard curve range: 2.7 - 2000 pg/mL
(CD25/IL-2Rα)
standard curve range: 21 - 15000 pg/mL
(BTLA)
standard curve range: 27 - 20000 pg/mL
(B7-H2/ICOSL)
standard curve range: 27 - 20000 pg/mL
(BCA-1/CXCL-13)
standard curve range: 3.4 - 2500 ng/mL
(B7-H3/CD276)
standard curve range: 3.4 - 2500 ng/mL
(Galectin-1)
standard curve range: 34.3 - 25000 pg/mL
(CD80/B7-1)
standard curve range: 342 - 250000 pg/mL
(E-Cadherin)
standard curve range: 4.1 - 3000 pg/mL
(TNFα)
standard curve range: 41 - 30000 pg/mL
(GITR)
standard curve range: 41 - 30000 pg/mL
(Galectin-3)
standard curve range: 412 - 300000 pg/mL
(LAG-3)
standard curve range: 412 - 300000 pg/mL
(TLR-2)
standard curve range: 5.5 - 4000 pg/mL
(CD137/4-1BB)
standard curve range: 5.5 - 4000 pg/mL
(CTLA-4/CD152)
standard curve range: 5.5 - 4000 pg/mL
(TIM-3)
standard curve range: 55 - 40000 pg/mL
(CD27)
standard curve range: 55 - 40000 pg/mL
(PD-1)
standard curve range: 6.9 - 5000 pg/mL
(PD-L2)
standard curve range: 8.2 - 6000 pg/mL
(GranzymeB)
inter-assay cv: <20%
intra-assay cv: <10%
technique(s)
multiplexing: suitable
compatibility
configured for Premixed
detection method
fluorometric (Luminex® xMAP®)
shipped in
wet ice
storage temp.
2-8°C
General description
MILLIPLEX® Mouse Immuno-Oncology Checkpoint Protein Panel 1 is a 28-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, cell culture supernatant, cell lysate, and tissue/tumor extract samples: 4-1BBL/TNFSF9, 5′-NT/CD73, B7-H2/ICOSL, B7-H3/CD276, BCA-1/CXCL-13, BTLA, CD137/4-1BB, CD226/DNAM-1, CD25/IL-2Rα, CD27, CD40, CD80/B7-1, CTLA-4/CD152, E-cadherin, Galectin-1, Galectin-3, GITR, Granzyme B, HVEM, IFNγ, IL-10, LAG-3, PD-1, PD-L1, PD-L2, TIM-3, TLR-2, and TNFα.
Panel Type: MAGNETIC Circulating Cancer
Specificity
Application
This is an overnight incubation assay (16-18 hours at 2-8°C).
A maximum of 25 μL per well 1:2 diluted serum or plasma can be used.
Tissue culture samples may be run neat or appropriately diluted in control medium.
Note: Best results using cell/tissue lysate samples may require diluting in assay buffer while testing similarly diluted lysis buffer for matrix effects.
Research Category: Cancer/Immuno-oncology
Analytes:
4-1BBL/TNFSF9
5′-NT/CD73
B7-H2/ICOSL
B7-H3/CD276
BCA-1/CXCL-13
BTLA
CD137/4-1BB
CD226/DNAM-1
CD25/IL-2Rα
CD27
CD40
CD80/B7-1
CTLA-4/CD152
E-cadherin
Galectin-1
Galectin-3
GITR
Granzyme B
HVEM
IFNγ
IL-10
LAG-3
PD-1
PD-L1
PD-L2
TIM-3
TLR-2
TNFα
Features and Benefits
Packaging
Other Notes
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Target Organs
Respiratory Tract
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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