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234167

Sigma-Aldrich

Anti-Collagen Type I Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-Collagen I Antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

mouse, human, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

target post-translational modification

unmodified

General description

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~115 kDa (doublet) type I collagen protein.
Recognizes type I collagen. Exhibits slight cross-reactivity with type III collagen.
This Anti-Collagen Type I Rabbit pAb is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of Collagen Type I.

Immunogen

Fetal Mouse Skin
purified, fetal mouse skin collagen type I

Application

Frozen Sections (1:30-1:60, see comments)

Immunoblotting (1:200-1:500)

Immunofluorescence (1:20-1:40)

Immunoprecipitation (1:20-1:60)

Warning

Toxicity: Standard Handling (A)

Physical form

Undiluted serum.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Romanos, G.E., et al. 1992. J. Periodont. Res. 27, 101.
Zimmermann, B., et al. 1992. Eur. Arch. Biol. 103, 93.
Schröter-Kermani, C., et al. 1991. Matrix 11, 428.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Kristin V T Engebretsen et al.
Journal of applied physiology (Bethesda, Md. : 1985), 114(8), 988-997 (2013-02-16)
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International journal of molecular sciences, 21(3) (2020-02-12)
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International journal of molecular medicine, 38(2), 513-520 (2016-06-18)
Hepatitis B virus (HBV)-associated glomerulo-nephritis is the most common extra-hepatic disorder occurring with hepatitis B virus infection. In the present study, we hypothesized that HBV X protein (HBx) may play a critical role in renal interstitial fibrosis, as HBx has been shown to induce epithelial-mesenchymal transition (EMT)
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Britta Husse et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 293(5), R1898-R1907 (2007-08-10)
Mechanical load and chemical factors as stimuli for the different pattern of the extracellular matrix (ECM) could be responsible for cardiac dysfunction. Since fibroblasts can both synthesize and degrade ECM, ventricular fibroblasts from adult rat hearts underwent cyclical mechanical stretch

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