Skip to Content
Merck
  • Effect of acute hyperketonemia on the cerebral uptake of ketone bodies in nondiabetic subjects and IDDM patients.

Effect of acute hyperketonemia on the cerebral uptake of ketone bodies in nondiabetic subjects and IDDM patients.

American journal of physiology. Endocrinology and metabolism (2002-06-18)
G Blomqvist, M Alvarsson, V Grill, G Von Heijne, M Ingvar, J O Thorell, S Stone-Elander, L Widén, K Ekberg
ABSTRACT

Using R-beta-[1-(11)C]hydroxybutyrate and positron emission tomography, we studied the effect of acute hyperketonemia (range 0.7-1.7 micromol/ml) on cerebral ketone body utilization in six nondiabetic subjects and six insulin-dependent diabetes mellitus (IDDM) patients with average metabolic control (HbA(1c) = 8.1 +/- 1.7%). An infusion of unlabeled R-beta-hydroxybutyrate was started 1 h before the bolus injection of R-beta-[1-(11)C]hydroxybutyrate. The time course of the radioactivity in the brain was measured during 10 min. For both groups, the utilization rate of ketone bodies was found to increase nearly proportionally with the plasma concentration of ketone bodies (1.0 +/- 0.3 micromol/ml for nondiabetic subjects and 1.3 +/- 0.3 micromol/ml for IDDM patients). No transport of ketone bodies from the brain could be detected. This result, together with a recent study of the tissue concentration of R-beta-hydroxybutyrate in the brain by magnetic resonance spectroscopy, indicate that, also at acute hyperketonemia, the rate-limiting step for ketone body utilization is the transport into the brain. No significant difference in transport and utilization of ketone bodies could be detected between the nondiabetic subjects and the IDDM patients.

MATERIALS
Product Number
Brand
Product Description

Supelco
Acetone, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
L-(+)-Lactic acid solution, 30% in H2O (by weight)
Supelco
Acetone, analytical standard
Sigma-Aldrich
Pyruvaldehyde solution, 40 wt. % in H2O
Sigma-Aldrich
Chromium, chips, thickness ~2 mm, 99.5%
Sigma-Aldrich
3-Hydroxybutyric acid, 95%
Sigma-Aldrich
Acetone, natural, ≥97%
Sigma-Aldrich
Dimethylamine, anhydrous, ≥99%
Sigma-Aldrich
Acetone, ≥99%, meets FCC analytical specifications
Sigma-Aldrich
Acetone, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Dodecanedioic acid, 99%
Sigma-Aldrich
1-Butanol, 99.9%
Supelco
1-Butanol, analytical standard
Sigma-Aldrich
Chromium, powder, 99.5%, −100 mesh
Sigma-Aldrich
Chromium, powder, ≥99% trace metals basis, <45 μm
Sigma-Aldrich
Chromium, chips, 99.995% trace metals basis
Sigma-Aldrich
1-Butanol, for molecular biology, ≥99%
Sigma-Aldrich
1-Butanol, anhydrous, 99.8%
Sigma-Aldrich
Glycerol solution, 83.5-89.5% (T)
Sigma-Aldrich
Acetone, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)
Sigma-Aldrich
1-Butanol, ACS reagent, ≥99.4%
Sigma-Aldrich
1-Butanol, suitable for HPLC, ≥99.7%
Sigma-Aldrich
1-Butanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)
Sigma-Aldrich
L-(+)-Lactic acid solution, meets analytical specification of Ph. Eur., BP, 88-92% total acid basis
Sigma-Aldrich
L-(+)-Lactic acid solution, in H2O, ≥85%
Sigma-Aldrich
Acetone, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
Sigma-Aldrich
Acetone, Laboratory Reagent, ≥99.5%
Sigma-Aldrich
Acetone, suitable for HPLC, ≥99.9%
Sigma-Aldrich
L-(+)-Lactic acid solution, ~40% in H2O
Sigma-Aldrich
Acetone, suitable for HPLC, ≥99.8%