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  • A ubiquitous chromatin opening element (UCOE) confers resistance to DNA methylation-mediated silencing of lentiviral vectors.

A ubiquitous chromatin opening element (UCOE) confers resistance to DNA methylation-mediated silencing of lentiviral vectors.

Molecular therapy : the journal of the American Society of Gene Therapy (2010-07-01)
Fang Zhang, Amy R Frost, Mike P Blundell, Olivia Bales, Michael N Antoniou, Adrian J Thrasher
ABSTRACT

DNA methylation may restrict the activity of gene transfer vectors due to inadvertent silencing. In P19 embryonic carcinoma cells in vitro, we found that transgene expression regulated by the SFFV LTR and EF1 alpha promoter declined rapidly within 16 days, but for A2UCOE derived from the human HNRPA2B1-CBX3 housekeeping gene locus, remained completely stable. Silencing correlated with extensive epigenetic methylation of CpG sites, whereas the A2UCOE was almost completely resistant. Linking of the A2UCOE upstream of the SFFV LTR protected this element from both DNA methylation and silencing. Analysis of engrafted hematopoietic cells in vivo transduced with the same vectors revealed a similar pattern. The A2UCOE displayed little or no methylation in either primary or secondary graft recipients, and gene expression profiles were highly conserved between the two groups. These studies provide convincing evidence that DNA methylation plays a direct role in regulating self-inactivating (SIN) lentiviral transgene expression, and that the stability of expression from the A2UCOE is, at least in part, due to methylation resistance. The A2UCOE therefore has considerable utility for gene therapy applications where reliable and sustained gene expression is desirable.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
UCOE® Single Expression Hygromycin Vector Set
Sigma-Aldrich
UCOE® Dual Expression Puromycin Vector Set
Sigma-Aldrich
UCOE® Intermediate Vector Set
Sigma-Aldrich
UCOE® Dual Expression Hygromycin Vector Set
Sigma-Aldrich
UCOE® Single Expression Puromycin Vector Set