MAK015
Glucose-6-Phosphate Dehydrogenase Activity Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
G-6-PD Activity Assay Kit
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About This Item
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usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
cancer; hematological disorder
storage temp.
−20°C
Gene Information
human ... H6PD(9563)
mouse ... H6PD(100198)
rat ... H6PD(298655)
General description
Glucose-6-phosphate dehydrogenase (G6PDH) catalyzes the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step of the pentose phosphate pathway (PPP). The PPP pathway critical for maintaining the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH) and for the production of pentose sugars. The NADPH produced is critical for redox regulation via the regeneration of GSH and for providing reducing equivalents for fatty acid biosynthesis. Deficiencies in G6PDH predisposes individuals to non-immune hemolytic anemia.
Application
Glucose-6-phosphate dehydrogenase assay kit has been used in the determination of glucose-6-phosphate dehydrogenase (G6PDH) activity and to measure PDH (pyruvate dehydrogenase).
Suitability
Suitable for the measurement of Glucose-6-phosphate dehydrogenase (G6PDH) activity in various tissues and cells.
Principle
In the glucose-6-phosphate dehydrogenase assay kit, glucose-6-phosphate is oxidized to generate a product which is specifically detected by colorimetric (450 nm) assay. The G6PDH Assay Kit can detect G6PDH activity as low as 0.04mU G6PDH per well.
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Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible corrosive hazardous materials
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