I4144
Interleukin-3 from mouse
IL-3, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
IL-3, mIL-3, Interleukin-3 from mouse
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About This Item
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biological source
mouse
Quality Level
recombinant
expressed in E. coli
Assay
≥98% (SDS-PAGE)
form
lyophilized powder
potency
0.01-0.1 ng/mL ED50/EC50
quality
endotoxin tested
mol wt
15.1 kDa
packaging
pkg of 10 and 50 μg
technique(s)
cell culture | mammalian: suitable
impurities
≤1.000 EU/μg
color
white to yellow
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Il3(16187)
General description
Interleukin-3 is a hematopoietic growth factor, which stimulates basophil development and triggers histamine, IL-4 and IL-6 release. It improves monocyte and macrophage activation and phagocytosis, enhances the secretory function of eosinophils. Interleukin-3 promotes endothelial cell proliferation and migration.
Application
Interleukin-3 from mouse has been used for the determination of 5-lipoxygenase product leukotriene C4 (LTC4) formation. It has also been used as a component of RPMI 1640 medium for cell transfection.
Biochem/physiol Actions
Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.
Physical form
Lyophilized with 0.5 mg of bovine serum albumin. Sterile filtered through a 0.2 μm filter.
Analysis Note
The biological activity is measured by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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